|NeuN (D4G4O) XP® Rabbit mAb 24307||20 µl||
||H M R||46-55||Rabbit IgG|
|GAP43 (D9C8) Rabbit mAb 8945||20 µl||
||H M R||38, 43||Rabbit IgG|
|MAP2 (D5G1) XP® Rabbit mAb 8707||20 µl||
||M R||75, 82, 280||Rabbit IgG|
|Neurofilament-L (C28E10) Rabbit mAb 2837||20 µl||
||H M R||70||Rabbit IgG|
|β3-Tubulin (D71G9) XP® Rabbit mAb 5568||20 µl||
||H M R||55||Rabbit IgG|
|Synaptophysin (D8F6H) XP® Rabbit mAb 36406||20 µl||
||H M R||38||Rabbit IgG|
|PSD95 (D27E11) XP® Rabbit mAb 3450||20 µl||
||H M R||95||Rabbit IgG|
|UCHL1 (D3T2E) XP® Rabbit mAb 13179||20 µl||
||H M R Mk||27||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody 7074||100 µl||
Rabbit monoclonal antibodies are produced by immunizing animals with one of the following antigens: 1) synthetic peptides corresponding to residues surrounding Lys30 of human GAP43 protein, Glu450 of human Neurofilament-L protein, Gly299 of human Synaptophysin protein, or Gln53 of human PSD95 protein, 2) synthetic peptides corresponding to residues near the carboxy terminus of human MAP2 protein, the carboxy terminus of human β3-tubulin protein, or the carboxy terminus of human UCHL1 protein, or 3) recombinant protein specific to the amino terminus of human NeuN protein.
The antibodies in this kit serve to characterize and identify mature neurons. Neural stem cells differentiate into mature post-mitotic neurons that are incapable of cellular division. Several neuron-enriched markers can be used to identify mature neurons. Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product, which contains an RNA recognition motif and functions as a splicing regulator (2). As neurons mature, they develop elaborate processes like axons and dendrites that are necessary to drive core neuronal functions, including synaptic transmission.
GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4). The cytoskeleton, which is important in generating neuronal processes, consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. β3-tubulin is one of six β-tubulin isoforms that make up the building blocks of microtubules (5). Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (6). MAP2 is preferentially localized to dendrites in cultured neurons (7). Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (8). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (9). Neurofilaments are critical for radial axon growth and determine axon caliber, serving as markers for neuronal axons.
Mature neurons function as cellular mediators of synaptic transmission. Synaptophysin is a neuronal synaptic vesicle glycoprotein (10). Synaptophysin is responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, and is a critical component and marker for the presynaptic fusion complex (11). Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. These family members consist of an amino-terminal variable segment followed by three PDZ domains, an SH3 domain, and an inactive guanylate kinase (GK) domain. PSD95 is a scaffolding protein involved in the assembly and function of mature postsynaptic density complexes (12,13).
Several cellular processes are required to support dynamic functions existing in mature neurons, including protein regulation by protein ubiquitination. Ubiquitin C-terminal hydrolase L1 (UCH-L1) is a deubiquitinating enzyme that is selectively and abundantly expressed in the brain, and its activity is required for normal synaptic function (14).
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