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Render Timestamp: 2024-12-13T11:13:14.696Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-10-10 21:56:07.799
Product last modified at: 2024-10-11T07:00:56.027Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

MCP-1 Antibody #2027

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Inquiry Info. # 2027

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    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 13-15
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MCP-1 Antibody detects endogenous levels of total human MCP-1 protein.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys35 of human MCP-1. Antibodies were purified by protein A and peptide affinity chromatography.

    Background

    Monocyte chemoattractant protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or β) chemokines (1-4). The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis (5), rheumatoid arthritis (6) and atherosclerosis (7). MCP-1 may also contribute to tumor progression and angiogenesis (8). Signaling by MCP-1 is mediated by the G protein-coupled receptor CCR2 (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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