Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human MEIS1 (hMEIS1-Myc/DDK; +) or MEIS2 (hMEIS2-Myc/DDK; +), using MEIS1/2 Antibody.
|MW (kDa)||50, 70|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
MEIS1/2 Antibody recognizes transfected levels of total MEIS1 and MEIS2 proteins.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MEIS1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Hox, Pbx, and Meis are families of transcription factors that bind DNA via their homeodomains. Members from each family form heterodimers to give rise to complexes with unique DNA binding specificities. Homeodomain containing proteins are frequently involved in normal developmental processes, but can also be associated with tumorigenic states (1). MEIS proteins belong to the TALE (Three Amino Acid Loop Extension) homeobox containing transcription factor family. MEIS1 has been associated with leukemogenesis and neuroblastoma (2,3) while MEIS2 is known to play an important role in the transcriptional program that is induced in normal pancreatic development (4) and cardiogenesis (5).
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