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8211
PhosphoPlus® MEK1/2 (Ser217/Ser221) Antibody Duet

PhosphoPlus® MEK1/2 (Ser217/Ser221) Antibody Duet #8211

Western Blotting Image 1

Western blot analysis of extracts from various cell lines using MEK1/2 (D1A5) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from untreated or TPA-treated HeLa and NIH/3T3 cells, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (upper), or MEK1/2 Antibody #9122 (lower).

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Flow Cytometry Image 3

Flow cytometric analysis of Jurkat cells using MEK1/2 (D1A5) Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

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IF-IC Image 4

Confocal immunofluorescent analysis of HeLa cells using MEK1/2 (D1A5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
MEK1/2 (D1A5) Rabbit mAb 8727 100 µl
  • WB
  • IF
  • F
H M R Mk Dm 45 Rabbit IgG
Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb 9154 100 µl
  • WB
  • IP
H M R Mk Dm 45 Rabbit 

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

  1. Crews, C.M. et al. (1992) Science 258, 478-480.
  2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19.
  3. Rosen, L.B. et al. (1994) Neuron 12, 1207-21.
  4. Cowley, S. et al. (1994) Cell 77, 841-52.
Entrez-Gene Id
5604 , 5605
Swiss-Prot Acc.
Q02750 , P36507
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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To Purchase # 8211S

Product Number Size Price
8211S 1 Kit (10 western blots) $472.00.0
Quantity Subtotal
$0.00