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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MESH1/HDDC3 (E2U8P) Rabbit mAb #64943

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 22
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MESH1/HDDC3 (E2U8P) Rabbit mAb recognizes endogenous levels of total MESH1/HDDC3 protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro36 of human MESH1/HDDC3 protein.

    Background

    MESH1 (Metazoan SpoT homolog 1), also known as HDDC3 (HD domain-containing protein 3), is an NADPH phosphatase in the cytosol. MESH1/HDDC3 is induced in stressed cells, depleting NADPH and stimulating ferroptosis (1). In addition, removal of MESH1/HDDC3 maintains NADPH levels in cells under stress conditions and therefore promotes ferroptotic cell survival (1). Furthermore, knockdown of MESH1/HDDC3 was shown to induce endoplasmic reticulum (ER) stress and integrative stress responses to facilitate cancer cell survival (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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