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MG-H1 Glycated Histone H3 (Arg17) (E3Z1R) Rabbit Monoclonal Antibody

MG-H1 Glycated Histone H3 (Arg17) (E3Z1R) Rabbit Monoclonal Antibody #94948

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	17
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IF-Immunofluorescence
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Immunocytochemistry)	1:3200
Flow Cytometry (Fixed/Permeabilized)	1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Methylglyoxal-derived hydroimidazolone 1 (MG-H1) is the most predominant of three primary methylglyoxal-derived hydroimidazolone isomers (MG-H1, MG-H2, and MG-H3) that form as advanced glycation end-products when methylglyoxal (MGO) non-enzymatically reacts with arginine residues on proteins.

MG-H1 Glycated Histone H3 (Arg17) (E3Z1R) Rabbit mAb detects endogenous MGO-derived glycation of the MG-H1 subtype at Arg17 of histone H3. This antibody may also weakly detect MG-H2 glycated Arg17 of histone H3. In addition, this antibody appears to weakly detect an additional MG-H1 glycated arginine residue on histone H3.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the N-terminal tail of histone H3 in which Arg17 is MG-H1 glycated.

Background

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, composed of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). Glycation is an additional posttranslational modification that occurs non-enzymatically within cells, involving the condensation of monosaccharides or glycolytic by-products with lysine or arginine residues (7). Methylglyoxal (MGO), a highly reactive by-product of glycolysis, readily binds with arginine to form the product methylglyoxal-derived hydroimidazolone (MG-H) in three possible isomeric forms: MG-H1, MG-H2, and MG-H3 (8). The accumulation of MGO-glycated proteins is readily observed in aging cells, individuals with hyperglycemia or diabetes, and various types of cancer (9,10). Notably, MGO-glycated histone H3 has been shown to accumulate in breast cancer cells and patient tumors, potentially contributing to genomic instability by modifying chromatin structure (11).

Alternate Names

H3; H3 clustered histone 1; H3 histone family, member A; H3/A; H31; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3FB; H3FC; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; Histone H3; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l

Database Links

UniProt ID: P68431

Entrez-Gene Id: 8350

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