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  3. MHC Class II (I-E alpha) (F8N7J) Rabbit Monoclonal Antibody (BSA and Azide Free)
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MHC Class II (I-E alpha) (F8N7J) Rabbit Monoclonal Antibody (BSA and Azide Free) #88714

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    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 32
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #91388. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #91388

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    MHC Class II (I-E alpha) (F8N7J) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MHC Class II (I-Eα) protein. This antibody does not cross-react with MHC Class II (I-Aα) protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val247 of mouse MHC Class II (I-Eα) protein.

    Background

    Major histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells, such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling (1). Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome (2). Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens (2). In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). HLAs corresponding to MHC class II are HLA-DP, HLA-DM, HLA-DOA, HLA-DOB, HLA-DQ, and HLA-DR (3).
    In mice, MHC class II molecules are encoded by the H2 gene complex, which includes two main isotypes: I-A and I-E. I-A and I-E are each composed of an α and β chain. The expression and polymorphism of these molecules vary between mouse strains, influencing immune responses, disease susceptibility, and compatibility in transplantation models (4). MHC Class II (I-Eα) pairs with the I-Eβ chain to form a heterodimer that is essential for antigen presentation to CD4+ T cells. MHC Class II (I-Eα) expression is strain-dependent, making it a valuable marker in immunology and transplantation studies (5,6).

    Alternate Names

    AI323765; E-alpha-f; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II, E-K alpha; H-2E; H-2Ea; H2-Ea; H2-Ea-ps; H2-Ea(K); H2-IE-alpha; HA22; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; I-E; I-E alpha MHC class II; I-Ealpha; Ia-3; Ia3; MHC class II alpha chain; MHC class II antigen E alpha; MHC class II antigen IEk-alpha; MHC class II H2-E alpha chain; MHC class II protein; MHC-H2-Ea

    Database Links

    UniProt ID: P04224


    Entrez-Gene Id: 100504404


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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