Render Target: STATIC
Render Timestamp: 2025-03-21T10:52:03.332Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:09:46.637
Product last modified at: 2025-01-01T09:04:14.632Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

MIS-R2 Antibody #4518

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Transfected Only
    MW (kDa) 75-85
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MIS-R2 Antibody detects transfected levels of human MIS-R2 protein.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu315 of human MIS-R2. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The type II receptor for Müllerian inhibiting substance (MIS), also known as the anti-Müllerian hormone receptor 2 (AMHR2), binds a hormone-ligand that directs the incomplete development of Müllerian ducts in male embryos (1,2). MIS-R2 is a single transmembrane serine/threonine kinase receptor of the TGF-β receptor family involved in the phosphorylation of shared type 1 receptors and Smad transcriptional regulators (3,4). MIS produced by the fetal testis promotes the regression of Müllerian ducts that would otherwise differentiate into the uterus and fallopian tubes in the male fetus (5). Corresponding MIS-R2 gene mutations can cause persistent Müllerian duct syndrome type 2 (PMDS-2), a form of male pseudohermaphroditism characterized by a failure of Müllerian duct regression (6). The presence of MIS-R2 is observed in ovarian cancer cell lines that respond positively to treatment with recombinant MIS, suggesting that both receptor and ligand may be important therapeutic tools (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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