MLL4 (F4A3R) Rabbit Monoclonal Antibody #36900
- WB
- IF
Product Specifications
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 593 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:800 - 1:3200 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:6400 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Mixed-lineage leukemia protein 4 (MLL4), also known as histone-lysine N-methyltransferase 2D (KMT2D), is a large 600 kDa protein that functions as part of the MLL4/COMPASS-like complex to activate gene expression by mediating mono-methylation of histone H3 lysine 4 at gene enhancers (7). Enhancer-specific H3 lysine 4 mono-methylation (H3K4me1) correlates with increased levels of chromatin interactions between gene enhancers and promoters, while loss of this modification results in a reduction of enhancer-promoter interactions (8). Furthermore, H3K4me1 facilitates recruitment of the cohesin complex, which may function to promote the interactions between gene enhancers and promoters (8). MLL4 is found to be mutated or have altered expression in various cancer types (9).
- Miller, T. et al. (2001) Proc Natl Acad Sci U S A 98, 12902-7.
- Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
- Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
- Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
- Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
- Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.
- Hu, D. et al. (2013) Mol Cell Biol 33, 4745-54.
- Yan, J. et al. (2018) Cell Res 28, 204-220.
- Sze, C.C. and Shilatifard, A. (2016) Cold Spring Harb Perspect Med 6,a026427. doi: 10.1101/cshperspect.a026427 .
Alternate Names
AAD10; ALL1-related protein; ALR; CAGL114; Histone-lysine N-methyltransferase 2D; Histone-lysine N-methyltransferase MLL2; KABUK1; Kabuki make-up syndrome; Kabuki mental retardation syndrome; KMS; KMT2D; lysine (K)-specific methyltransferase 2D; lysine methyltransferase 2D; Lysine N-methyltransferase 2B; Lysine N-methyltransferase 2D; MLL2; MLL4; myeloid/lymphoid or mixed-lineage leukemia 2; Myeloid/lymphoid or mixed-lineage leukemia protein 2; TNRC21; trinucleotide repeat containing 21; truncated lysine methyltransferase 2D
Limited Uses
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