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62860
PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet #62860

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other Image 1 - PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet

Western blot analysis of extracts from various cell lines using MOB1 (E1N9D) Rabbit mAb.

other Image 2 - PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet

Immunoprecipitation of MOB1 protein from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or MOB1 (E1N9D) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MOB1 (E1N9D) Rabbit mAb.

other Image 3 - PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet

Western blot analysis of extracts from MCF7 cells, either untreated (-) or treated (+) with H2O2 (2.5 mM, 30 min) and Ramos cells, either untreated (-) or treated (+) with λ phosphatase, using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb (upper) and MOB1 Antibody #3863 (lower).

other Image 4 - PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb.

other Image 5 - PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma control (left) or lambda phosphatase-treated (right) using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb.

other Image 6 - PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet

Immunohistochemical analysis of paraffin-embedded MCF7 cell pellets, control (left) or H2O2-treated (right), using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb.

To Purchase # 62860S
Product # Size Price
62860S
1 Kit $ 514

Product Includes Quantity Reactivity MW(kDa) Isotype
MOB1 (E1N9D) Rabbit mAb 13730 100 µl H M R Hm Mk 24 Rabbit IgG
Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb 8699 100 µl H M R Mk 24 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy (1). Its Drosophila and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and which has been implicated in cancer development (2-5). There are two MOB1 proteins in humans, MOB1A and MOB1B, that are encoded by two different genes but which have greater than 95% amino acid sequence identity (6). Both forms bind to members of the nuclear Dbf2-related (NDR) kinases, such as LATS1/2 and NDR1/2, thereby stimulating kinase activity (7-9). This binding is promoted by the phosphorylation of MOB1 at several threonine residues (e.g., Thr12, Thr35) by MST1 and/or MST2 (5,10).

  1. Luca, F.C. and Winey, M. (1998) Mol Biol Cell 9, 29-46.
  2. Edgar, B.A. (2006) Cell 124, 267-73.
  3. Saucedo, L.J. and Edgar, B.A. (2007) Nat Rev Mol Cell Biol 8, 613-21.
  4. Harvey, K. and Tapon, N. (2007) Nat Rev Cancer 7, 182-91.
  5. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
  6. Praskova, M. et al. (2008) Curr Biol 18, 311-21.
  7. Devroe, E. et al. (2004) J Biol Chem 279, 24444-51.
  8. Hergovich, A. et al. (2005) Mol Cell Biol 25, 8259-72.
  9. Hergovich, A. et al. (2006) Biochem Biophys Res Commun 345, 50-8.
  10. Hirabayashi, S. et al. (2008) Oncogene 27, 4281-92.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.