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62860
PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet #62860

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PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet: Image 1
Western blot analysis of extracts from various cell lines using MOB1 (E1N9D) Rabbit mAb.
PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet: Image 2
Western blot analysis of extracts from MCF7 cells, either untreated (-) or treated (+) with H2O2 (2.5 mM, 30 min) and Ramos cells, either untreated (-) or treated (+) with λ phosphatase, using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb (upper) and MOB1 Antibody #3863 (lower).
PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet: Image 3
Immunoprecipitation of MOB1 protein from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or MOB1 (E1N9D) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MOB1 (E1N9D) Rabbit mAb.
PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet: Image 4
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb.
PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet: Image 5
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma control (left) or lambda phosphatase-treated (right) using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb.
PhosphoPlus® MOB1A/MOB1B (Thr35) Antibody Duet: Image 6
Immunohistochemical analysis of paraffin-embedded MCF7 cell pellets, control (left) or H2O2-treated (right), using Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb.
To Purchase # 62860S
Product # Size Price
62860S
1 Kit $ 529

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy (1). Its Drosophila and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and which has been implicated in cancer development (2-5). There are two MOB1 proteins in humans, MOB1A and MOB1B, that are encoded by two different genes but which have greater than 95% amino acid sequence identity (6). Both forms bind to members of the nuclear Dbf2-related (NDR) kinases, such as LATS1/2 and NDR1/2, thereby stimulating kinase activity (7-9). This binding is promoted by the phosphorylation of MOB1 at several threonine residues (e.g., Thr12, Thr35) by MST1 and/or MST2 (5,10).
  1. Luca, F.C. and Winey, M. (1998) Mol Biol Cell 9, 29-46.
  2. Edgar, B.A. (2006) Cell 124, 267-73.
  3. Saucedo, L.J. and Edgar, B.A. (2007) Nat Rev Mol Cell Biol 8, 613-21.
  4. Harvey, K. and Tapon, N. (2007) Nat Rev Cancer 7, 182-91.
  5. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
  6. Praskova, M. et al. (2008) Curr Biol 18, 311-21.
  7. Devroe, E. et al. (2004) J Biol Chem 279, 24444-51.
  8. Hergovich, A. et al. (2005) Mol Cell Biol 25, 8259-72.
  9. Hergovich, A. et al. (2006) Biochem Biophys Res Commun 345, 50-8.
  10. Hirabayashi, S. et al. (2008) Oncogene 27, 4281-92.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.