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MORC2 Antibody #77667

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  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 135
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MORC2 Antibody recognizes endogenous levels of total MORC2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu681 of human MORC2 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Microrchidia family CW-type zinc finger 2 (MORC2) is a protein involved in ATP-dependent chromatin remodeling and DNA repair (1-3). Upon DNA damage, MORC2 dimerizes and is phosphorylated by PAK1. PARP1 can then recruit MORC2 to break sites, where it destabilizes histone-DNA interactions, and recruits DNA repair proteins, such as BRCA1 and Rad51 (4,5). MORC2 is recruited by the HUSH epigenetic silencing complex to sites of heterochromatin (6). MORC2 has also been shown to downregulate important tumor suppressors p21 and ArgBP2 in gastric cancers by recruitment of EZH2 (7-9). Mutations in the MORC2 gene locus have been shown to cause the axonal form of Charcot-Marie-Tooth disease (CMT), a disorder of the motor and sensory neurons of the peripheral nervous system (8-11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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