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37988
Mouse (E1D5H) mAb IgG3 Isotype Control

Mouse (E1D5H) mAb IgG3 Isotype Control #37988

APPLICATIONS

Concentration SENSITIVITY Isotype
2.5 mg/ml Mouse IgG3
IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Histone H3 (1B1B2) Mouse mAb #14269 (left) compared to concentration-matched Mouse (E1D5H) mAb IgG3 Isotype Control (right).

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IF-IC

Confocal immunofluorescent analysis of SNB-19 cells using Histone H3 (1B1B2) Mouse mAb #14269 (left, green) compared to concentration-matched Mouse (E1D5H) mAb IgG3 Isotype Control (right, green). Cells were counterstained with β-Actin (13E5) Rabbit mAb #4970 (red).

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Flow Cytometry

Flow cytometric analysis of K-562 cells using Histone H3 (1B1B2) Mouse mAb #14269 (solid line) compared to concentration-matched Mouse (E1D5H) mAb IgG3 Isotype Control (dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

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Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MEF cells treated with tunicamycin (2 μg/ml, 10 hr) and either Mouse (E1D5H) mAb IgG3 Isotype Control, Normal Rabbit IgG #2729, or Histone H3 (1B1B2) Mouse mAb #14269 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse ATF-3 Intron 1 Primers #13059, mouse CHOP promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions for Use.

Important! Dilute this control antibody to the same concentration (not dilution) as the specific antibody used for analysis. Higher background may result if excessive amounts of mouse IgG3 isotype control are used.

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Mouse (E1D5H) mAb IgG3 Isotype Control is not directed against any known antigen. It functions as an isotype control for mouse IgG3 monoclonal antibodies.

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.

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To Purchase # 37988S

Product Number Size Price
37988S 100 µl $113.00.0
Quantity Subtotal
$0.00