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61656
Mouse (E5Y6Q) mAb IgG2a Isotype Control

Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656

APPLICATIONS

Concentration SENSITIVITY Isotype
2.5 mg/ml Mouse IgG2a
IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using PCNA (PC10) Mouse mAb (left) compared to concentration matched Mouse (E5Y6Q) mAb IgG2a Isotype Control (right).

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IF-IC

Confocal immunofluorescent analysis of HCT 116 cells using CD44 (156-3C11) Mouse mAb #3570 (left, green) compared to concentration matched Mouse (E5Y6Q) mAb IgG2a Isotype Control (right, green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

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Flow Cytometry

Flow cytometric analysis of unpermeabilized HeLa cells using CD44 (156-3C11) Mouse mAb #3570 (solid line) compared to concentration-matched Mouse (E5Y6Q) mAb IgG2a Isotype Control (dashed line). Anti-mouse IgG (H+L) F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

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Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 mouse embryonic fibroblasts treated with tunicamycin (2ug/ml, 10hr) and either Mouse (E5Y6Q) mAb IgG2a Isotype Control, Normal Rabbit IgG #2729, or CHOP (L63F7) Mouse mAb #2895 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse ATF-3 Intron 1 Primers #13059, mouse CHOP promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions for Use.

Important! Dilute this control antibody to the same concentration (not dilution) as the specific antibody used for analysis. Higher background may result if excessive amounts of mouse IgG2a isotype control are used.

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Mouse (E5Y6Q) mAb IgG2a Isotype Control is not directed against any known antigen. It functions as an isotype control for mouse IgG2a monoclonal antibodies.

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.

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To Purchase # 61656S

Product Number Size Price
61656S 100 µl $113.00.0
Quantity Subtotal
$0.00