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53484
Mouse (E7Q5L) mAb IgG2b Isotype Control
Primary Antibodies
Monoclonal Antibody

Mouse (E7Q5L) mAb IgG2b Isotype Control #53484

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  1. IP
  2. IHC
  3. IF
  4. ChIP
Immunoprecipitation Image 1: Mouse (E7Q5L) mAb IgG2b Isotype Control

Immunoprecipitation of IDH2 from Jurkat cell extracts. Lane 1 is 10% input, lane 2 is Mouse (E7Q5L) mAb IgG2b Isotype Control, and lane 3 is IDH2 (KrMab-3) Mouse mAb #60322. Western blot analysis was performed using IDH2 (D8E3B) Rabbit mAb #56439. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.

Immunohistochemistry Image 1: Mouse (E7Q5L) mAb IgG2b Isotype Control

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using p53 (DO-7) Mouse mAb #48818 (left) compared to concentration matched Mouse (E7Q5L) mAb IgG2b Isotype Control (right).

Immunofluorescence Image 1: Mouse (E7Q5L) mAb IgG2b Isotype Control

Confocal immunofluorescent analysis of HCT 116 cells using β-Actin (8H10D10) Mouse mAb #3700 (left, green) compared to concentration matched Mouse (E7Q5L) mAb IgG2b Isotype Control (right, green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

Chromatin Immunoprecipitation Image 1: Mouse (E7Q5L) mAb IgG2b Isotype Control

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT116 cells treated with UV (1000 J/m2 followed by a 3 hour recovery) and either Mouse (E7Q5L) mAb IgG2b Isotype Control, Normal Rabbit IgG #2729, or p53 (DO-7) Mouse mAb #48818 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1A Promoter Primers #6449, SimpleChIP® Human MDM2 Intron 2 Primers #90678, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

To Purchase # 53484S
Product # Size Price
53484S
100 µl $ 121

Supporting Data

Concentration 2.5 mg/ml
SENSITIVITY
Source/Isotype Mouse IgG2b

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used in analysis. Higher background fluorescence may result if excessive amounts of isotype control are used. For protocol details, please reference the product page for the specific antibody used in analysis.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Mouse (E7Q5L) mAb IgG2b Isotype Control is not directed against any known antigen. It functions as an isotype control for mouse IgG2b monoclonal antibodies.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.