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53484
Mouse (E7Q5L) mAb IgG2b Isotype Control

Mouse (E7Q5L) mAb IgG2b Isotype Control #53484

APPLICATIONS

Concentration SENSITIVITY Isotype
2.5 mg/ml Mouse IgG2b
IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using p53 (DO-7) Mouse mAb #48818 (left) compared to concentration matched Mouse (E7Q5L) mAb IgG2b Isotype Control (right).

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IF-IC

Confocal immunofluorescent analysis of HCT 116 cells using β-Actin (8H10D10) Mouse mAb #3700 (left, green) compared to concentration matched Mouse (E7Q5L) mAb IgG2b Isotype Control (right, green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

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Flow Cytometry

Flow cytometric analysis of HeLa cells using β-Actin (8H10D10) Mouse mAb #3700 (solid line) compared to concentration-matched Mouse (E7Q5L) mAb IgG2b Isotype Control (dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

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Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT116 cells treated with UV (1000 J/m2 followed by a 3 hour recovery) and either Mouse (E7Q5L) mAb IgG2b Isotype Control, Normal Rabbit IgG #2729, or p53 (DO-7) Mouse mAb #48818 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1A Promoter Primers #6449, SimpleChIP® Human MDM2 Intron 2 Primers #90678, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions for Use.

Important! Dilute this control antibody to the same concentration (not dilution) as the specific antibody used for analysis. Higher background may result if excessive amounts of mouse IgG2b isotype control are used.

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Mouse (E7Q5L) mAb IgG2b Isotype Control is not directed against any known antigen. It functions as an isotype control for mouse IgG2b monoclonal antibodies.

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.

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To Purchase # 53484S

Product Number Size Price
53484S 100 µl $113.00.0
Quantity Subtotal
$0.00