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97624
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit #97624

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Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 1
Western blot analysis of extracts from various cell lines using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 2
Western blot analysis of extracts from J774A.1 cells, Neuro-2a cells, and mouse spleen tissue using CD86 (E5W6H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 3
Western blot analysis of extracts from various cell lines and tissues using CD206/MRC1 (E6T5J) XP® Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 4
Western blot analysis of extracts from various cell lines using F4/80 (D2S9R) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 5
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 6
Western blot analysis of extracts from mouse and rat liver using Arginase-1 (D4E3M) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 7
Western blot analysis of extracts from Raw 264.7, C2C12, and 3T3 cells using CD11c (D1V9Y) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 8
Western blot analysis of extracts from various mouse cells using CD68 (E3O7V) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 9
Immunoprecipitation of Iba1/AIF-1 from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Iba1/AIF-1 (E4O4W) XP® Rabbit mAb. Western blot analysis was performed using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 10
Immunohistochemical analysis of paraffin-embedded mouse liver using CD86 (E5W6H) Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 11
Immunoprecipitation of CD206/MRC1 protein from mouse liver extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CD206/MRC1 (E6T5J) XP® Rabbit mAb. Western blot analysis was performed using CD206/MRC1 (E6T5J) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 12
Immunohistochemical analysis of paraffin-embedded mouse liver using F4/80 (D2S9R) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 13
Western blot analysis of extracts from mouse liver and mouse small intestine using Arginase-1 (D4E3M) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 14
Immunohistochemical analysis of paraffin-embedded 4T1 mammary tumor using CD11c (D1V9Y) Rabbit mAb performed on the Leica® Bond Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 15
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse CD68 protein (mCD68-Myc/DDK; +), using CD68 (E3O7V) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 16
Immunohistochemical analysis of paraffin-embedded human neuroendocrine carcinoma of the lung using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 17
Immunohistochemical analysis of paraffin-embedded mouse lung using CD86 (E5W6H) Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 18
Immunohistochemical analysis of paraffin-embedded mouse liver using CD206/MRC1 (E6T5J) XP® Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 19
Immunohistochemical analysis of paraffin-embedded mouse spleen using F4/80 (D2S9R) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 20
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Arginase-1 (D4E3M) XP® Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded mouse kidney using CD11c (D1V9Y) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 22
Immunohistochemical analysis of paraffin-embedded mouse colon using CD68 (E3O7V) Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 23
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 24
Immunohistochemical analysis of paraffin-embedded CT26.WT syngeneic tumor using CD86 (E5W6H) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 25
Immunohistochemical analysis of paraffin-embedded mouse lung using CD206/MRC1 (E6T5J) XP® Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 26
Immunohistochemical analysis of paraffin-embedded mouse small intestine using F4/80 (D2S9R) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 27
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Arginase-1 (D4E3M) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 28
Immunohistochemical analysis of paraffin-embedded mouse liver using CD11c (D1V9Y) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 29
Immunohistochemical analysis of paraffin-embedded mouse lung using CD68 (E3O7V) Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 30
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (left) or Iba1/AIF-1 (E5N4J) Mouse mAb (IHC Formulated) #58970 (right) performed on the Leica® BOND Rx. These two antibodies detect independent, unique epitopes on human Iba1/AIF-1 protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 31
Immunohistochemical analysis of paraffin-embedded GL-261 syngeneic tumor using CD86 (E5W6H) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 32
Immunohistochemical analysis of paraffin-embedded mouse stomach using CD206/MRC1 (E6T5J) XP® Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 33
Immunohistochemical analysis of paraffin-embedded Raw 264.7 cell pellet (left, positive) or Neuro-2A cell pellet (right, negative) using F4/80 (D2S9R) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 34
Immunohistochemical analysis of paraffin-embedded normal human liver using Arginase-1 (D4E3M) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 35
Immunohistochemical analysis of paraffin-embedded mouse lung using CD11c (D1V9Y) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 36
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD68 (E3O7V) Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 37
Immunohistochemical analysis of paraffin-embedded mouse brain using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 38
Immunohistochemical analysis of paraffin-embedded J774A.1 cell pellet (left, positive) or BA/F3 cell pellet (right, negative) using CD86 (E5W6H) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 39
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using CD206/MRC1 (E6T5J) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 40
Multiplex immunohistochemical analysis of paraffin-embedded mouse LL2 syngeneic tumor tissue using F4/80 (D2S9R) XP® rabbit mAb (orange), PD-1 (D7D5W) XP® rabbit mAb #84651 (green), PD-L1 (D5V3B) rabbit mAb #64988 (yellow), CD3ε (D4V8L) rabbit mAb #99940 (red), CD8α (D4W2Z) XP® rabbit mAb #98941 (magenta) and pan keratin (C11) mouse mAb #4279 (cyan).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 41
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Arginase-1 (D4E3M) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 42
Immunohistochemical analysis of paraffin-embedded Raw 264.7 cell pellet (left, positive) or C2C12 cell pellet (right, negative) using CD11c (D1V9Y) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 43
Immunohistochemical analysis of paraffin-embedded mouse testis using CD68 (E3O7V) Rabbit mAb performed on the Leica® BOND Rx.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 44
Immunohistochemical analysis of paraffin-embedded THP-1 cell pellet (left, positive) or SH-SY5Y cell pellet (right, negative) using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 45
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD86 (E5W6H) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 46
Immunohistochemical analysis of paraffin-embedded CT26.WT syngeneic tumor using CD206/MRC1 (E6T5J) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 47
Immunohistochemical analysis of paraffin-embedded mouse liver using Arginase-1 (D4E3M) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 48
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD11c (D1V9Y) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 49
Immunohistochemical analysis of paraffin-embedded RAW 264.7 cell pellet (left, positive), Renca cell pellet (middle, positive), or Neuro-2a cell pellet (right, negative) using CD68 (E3O7V) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 50
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 51
Immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using CD206/MRC1 (E6T5J) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 52
Confocal immunofluorescent analysis of mouse spleen (left) and mouse kidney (right) using CD11c (D1V9Y) Rabbit mAb. Nuclei were labeled with ProLong® Gold Antifade reagent with DAPI #8961 (blue).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 53
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD68 (E3O7V) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 54
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 55
Immunohistochemical analysis of paraffin-embedded mouse colon using CD206/MRC1 (E6T5J) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 56
Confocal immunofluorescent analysis of mouse liver (positive; left) or small intestine (negative; right) using Arginase-1 (D4E3M) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5 #4084 (fluorescent DNA dye).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 57
Confocal immunofluorescent analysis of Raw 264.7 cells (left, positive) and 3T3 cells (right, negative) using CD11c (D1V9Y) Rabbit mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 58
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using CD68 (E3O7V) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 59
Immunohistochemical analysis of paraffin-embedded mouse small intestine using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 60
Immunohistochemical analysis of paraffin-embedded mouse pancreas using CD206/MRC1 (E6T5J) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 61
Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) using Arginase-1 (D4E3M) XP® Rabbit mAb (green). BMDMs were differentiated with M-CSF (20 ng/ml, 7 days) and activated with either IL-4/cAMP (20 ng/ml, 0.5 mM, 24 hours; left) or LPS/IFNγ (50 ng/ml, 20 ng/ml, 24 hours; right). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 62
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using CD68 (E3O7V) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 63
Immunohistochemical analysis of paraffin-embedded mouse spleen using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 64
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD206/MRC1 (E6T5J) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 65
Flow cytometric analysis of human whole blood using Arginase-1 (D4E3M) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Analysis was performed on cells in the granulocyte gate.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 66
Immunohistochemical analysis of paraffin-embedded mouse liver using CD68 (E3O7V) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 67
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 68
Immunohistochemical analysis of paraffin-embedded mouse testis using CD206/MRC1 (E6T5J) XP® Rabbit mAb (left) or CD206/MRC1 antibody (right). These two antibodies detect independent, unique epitopes on mouse CD206/MRC1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 69
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using CD68 (E3O7V) Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 70
Confocal immunofluorescent analysis of human cortex (left) and mouse CA1 hippocampus (right) using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (green). In mouse tissue sections, cell nuclei were labeled with DAPI (blue). Images kindly provided by Dr. Simone Brioschi and Dr. Marco Colonna (Washington University) and used with permission.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 71
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using CD206/MRC1 (E6T5J) XP® Rabbit mAb.
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 72
Confocal immunofluorescent analysis of mouse colon (left) and liver (right) using CD68 (E3O7V) Rabbit mAb (red). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) (Alexa Fluor® 488 Conjugate) #68206 (green). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 73
Confocal immunofluorescent analysis of mouse small intestine using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 74
Confocal immunofluorescent analysis of mouse heart using CD206/MRC1 (E6T5J) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 650 Phalloidin #12956 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 75
Confocal immunofluorescent analysis of RAW 264.7 cells (left, positive) and Neuro-2a cells (right, negative) using CD68 (E3O7V) Rabbit mAb (green). Cells were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 76
Confocal immunofluorescent analysis of mouse liver using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 77
Confocal immunofluorescent analysis of mouse spleen using CD206/MRC1 (E6T5J) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 650 Phalloidin #12956 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 78
Confocal immunofluorescent analysis of microglia in mouse hippocampus using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (green). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 79
Confocal immunofluorescent analysis of mouse liver using CD206/MRC1 (E6T5J) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 650 Phalloidin #12956 (red). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 80
Confocal immunofluorescent analysis of THP-1 cells differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, 24 hr; left, positive) and SH-SY5Y cells (right, negative), using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit: Image 81
Flow cytometric analysis of SH-SY5Y cells (blue, negative) and THP-1 cells (green, positive) using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
To Purchase # 97624
Cat. # Size Qty. Price
97624T
1 Kit  (6 x 20 microliters) $ 534

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
F4/80 (D2S9R) XP® Rabbit mAb 70076 20 µl
  • WB
  • IP
  • IHC
M 65-250 Rabbit IgG
CD68 (E3O7V) Rabbit mAb 97778 20 µl
  • WB
  • IHC
  • IF
M 70-80, 130-140, 200 Rabbit IgG
CD86 (E5W6H) Rabbit mAb 19589 20 µl
  • WB
  • IHC
M 60-85 Rabbit IgG
CD11c (D1V9Y) Rabbit mAb 97585 20 µl
  • WB
  • IHC
  • IF
M 145 Rabbit IgG
CD206/MRC1 (E6T5J) XP® Rabbit mAb 24595 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R 190-250 Rabbit IgG
Arginase-1 (D4E3M) XP® Rabbit mAb 93668 20 µl
  • WB
  • IHC
  • IF
  • F
H M R 40 Rabbit IgG
Iba1/AIF-1 (E4O4W) XP® Rabbit mAb 17198 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R 17 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit provides an economical means of characterizing the extent of M1 and M2 macrophages in formalin-fixed, paraffin-embedded tissue samples.

Specificity / Sensitivity

Each antibody in the Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit detects endogenous levels of its target human protein. Arginase-1 (D4E3M™) XP® Rabbit mAb does not cross-react with arginase-2. CD206/MRC1 (E6T5J) XP® Rabbit mAb recognizes mouse CD206/MRC1 protein and is also reactive with human CD206/MRC1; however, this antibody is not suggested for immunohistochemical analysis of human tissues. Instead, CD206/MRC1 (E2L9N) Rabbit mAb #91992 is recommended for IHC analysis of human tissue samples. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry using CD68 (E3O7V) Rabbit mAb.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of mouse CD206/MRC1 protein and surrounding Ala1153 of mouse CD11c protein, Val47 of human arginase-1 protein, and Ala139 of human Iba1/AIF-1 protein, or with recombinant proteins specific to mouse F4/80, CD86, and CD68.

Background

Macrophages are myeloid cells of the innate immune system that are found in all human tissues in the body and exhibit anatomical and functional diversity. These heterogenous cells are derived from monocyte precursors in the blood that infiltrate into the tissues and differentiate in the presence of cytokines and growth factors. A spectrum of different macrophage phenotypes, or polarizations, have been described based on their secretory profiles, gene expression, and functions. Macrophages have great plasticity and can switch from one phenotype to another under different conditions. At the opposite extremes of this spectrum are so called M1, or classically activated phenotype, and M2 or alternatively activated phenotype. M1 macrophages are generally inflammatory and anti-tumor, while M2 macrophages, commonly referred to as tumor-associated macrophages (TAMs), are generally anti-inflammatory and pro-tumor. Relative contents of M1 and M2 macrophages in the tumor microenvironment may have prognostic values. Modulating macrophage polarization is actively pursued as a therapeutic intervention for many different diseases (1-6).
In mice, F4/80, CD68, and Iba1/AIF-1 are considered general markers for macrophages. CD86, CD11c, and others have been used as markers for M1 macrophages, while CD206, Arginase-1, and others have been used as markers for M2 macrophages (7-10).
  1. Wynn, T.A. et al. (2013) Nature 496, 445-55.
  2. Biswas, S.K. and Mantovani, A. (2010) Nat Immunol 11, 889-96.
  3. Mills, C.D. (2012) Crit Rev Immunol 32, 463-88.
  4. Wang, N. et al. (2014) Front Immunol 5, 614.
  5. Orecchioni, M. et al. (2019) Front Immunol 10, 1084.
  6. Yunna, C. et al. (2020) Eur J Pharmacol 877, 173090.
  7. Kim, H.M. et al. (2017) PLoS One 12, e0179204.
  8. Gensel, J.C. et al. (2017) Sci Rep 7, 40144.
  9. Li, X. et al. (2018) Front Pharmacol 9, 72.
  10. Yang, J. et al. (2018) Cereb Cortex 28, 2622-2635.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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