Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit #85741
Product Information
Kit Usage Information
Protocols
- 2989: Western Blotting, Immunoprecipitation (Magnetic)
- 7074: Western Blotting
- 7076: Western Blotting
- 11948: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Flow
- 12242: Western Blotting, Immunohistochemistry (Paraffin)
- 12912: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow
- 27535: Western Blotting
- 41987: Western Blotting
- 87809: Western Blotting, Immunoprecipitation (Agarose)
Product Description
Specificity / Sensitivity
Each antibody in the Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit detects endogenous levels of its target protein. RANTES Antibody (Rodent Specific) detects transfected levels of rodent RANTES.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with recombinant human IL-1β protein, recombinant mouse TNF-α protein, recombinant mouse IL-6 protein, a synthetic peptide corresponding to residues surrounding Cys75 of mouse MCP-1 protein, or a synthetic peptide corresponding to residues surrounding Pro117 of human MMP-2 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of rodent RANTES protein or corresponding to residues surrounding Gly196 of murine PAI-1 protein. Polyclonal antibodies are purified by peptide affinity chromatography.
Background
Senescence is characterized by stable stress-induced proliferative arrest and resistance to mitogenic stimuli, as well as the secretion of proteins such as cytokines, growth factors, and proteases. These secreted proteins comprise the senescence-associated secretory phenotype (SASP). Senescent cells are thought to accumulate as an organism ages, and contribute to age-related diseases, including cancer, through promotion of inflammation and disruption of normal cellular function (1,2). The composition of the SASP varies, and SASP components can be either beneficial or deleterious in human disease, depending on the context (3).
The Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides a collection of antibodies to various SASP components, including TNF-α, interleukin-6 (IL-6), the multifunctional cytokine IL-1β, the chemokines RANTES/CCL5 and MCP-1, the matrix metalloprotease MMP-2, and the serine-protease inhibitor PAI-1.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.