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Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit
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Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit #85741

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Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 1
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated (+) with LPS (100 ng/mL, 6 hr) and Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation), using TNF-α (D2D4) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 2
Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/mL, 3 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 3
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of stimulation; +), using IL-6 (D5W4V) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 4
Western blot analysis of extracts from mouse placenta and mouse spleen using PAI-1 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The differential in PAI-1 expression between placenta and spleen is consistent with their reported RNA expression profiles (www.proteinatlas.org), confirming specificity of the antibody for PAI-1.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 5
Western blot analysis of HeLa cells, mock transfected or transfected with mouse CCL5/RANTES, using CCL5/RANTES Antibody.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 6
Western blot analysis of extracts from RAW 264.7 cells, untreated (-) or treated with LPS #14011 (1 μg/ml, overnight; +), using MCP-1 (E9R7Z) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 7
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 8
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 9
Western blot analysis of extracts from U87, 3T3-L1 and C2C12 cells using MMP-2 (D4O4T) Rabbit mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 10
Western blot analysis of 1 ng recombinant mouse TNF-α #5178 and 1 ng recombinant rat TNF-α using TNF-α (D2D4) XP® Rabbit mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 11
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation; +) and LPS (100 ng/mL, 6 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 12
Western blot analysis of recombinant Mouse His6Interleukin-6 (mHis6IL-6) using IL-6 (D5W4V) XP® Rabbit mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 13
Immunoprecipitation of MMP-2 protein from U87 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MMP-2 (D2O4T) Rabbit mAb. Western blot analysis was performed using MMP-2 (D2O4T) Rabbit mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 14
Immunoprecipitation of TNF-α from Raw 264.7 cell extracts, treated with LPS (100 ng/mL, 6 hr) and Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TNF-α (D2D4) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TNF-α (D2D4) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) #3678 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as sencondary antibodies.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 15
Western blot analysis of 1 ng recombinant Human Interleukin-1β (hIL-1β) #8900 and 1 ng recombinant Mouse Interleukin-1β (mIL-1β) #5204 using IL-1β (3A6) Mouse mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 16
Immunoprecipitation of IL-6 from Raw 264.7 cell extracts treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of stimulation) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IL-6 (D5W4V) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IL-6 (D5W4V) XP® Rabbit mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 17
Confocal immunofluorescent analysis of Raw 264.7 cells, treated with LPS (100 ng/mL, 6 hr; left) or untreated (right), using TNF-α (D2D4) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 18
Immunohistochemical analysis of paraffin-embedded human large intestine (chronic colitis of the colon) using IL-1-β (3A6) Mouse mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 19
Confocal immunofluorescent analysis of Raw 264.7 cells, untreated (left) or treated with LPS (100 ng/ml, 6 hr; right), using IL-6 (D5W4V) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 20
Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of treatment) (green), using TNF-α (D2D4) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded human large intestine (ulcerative chronic colitis of the rectum) using IL-1-β (3A6) Mouse mAb.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 22
Flow cytometric analysis of Raw 264.7 cells, treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of LPS treatment) (green) or Brefeldin A alone (300 ng/ml, 3 hr) (blue) using IL-6 (D5W4V) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit: Image 23
Immunohistochemical analysis of paraffin embedded THP-1 cell pellets, control (left) or LPS-treated (right) using IL-1-β (3A6) Mouse mAb.
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To Purchase # 85741
Cat. # Size Qty. Price
85741T		 1 Kit  (6 x 20 microliters)
$ 581

Custom Formulation

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
IL-1β (3A6) Mouse mAb 12242 20 µl
  • WB
  • IHC
 H M 17,31 Mouse IgG1
CCL5/RANTES Antibody 2989 20 µl
  • WB
  • IP
 M 10 Rabbit 
PAI-1 Antibody 27535 20 µl
  • WB
 M R 50 Rabbit 
TNF-α (D2D4) XP® Rabbit mAb 11948 20 µl
  • WB
  • IP
  • IF
  • F
 M 17,25,28 Rabbit IgG
MCP-1 (E9R7Z) Rabbit mAb 41987 20 µl
  • WB
 M 11-13, 20-30 Rabbit IgG
MMP-2 (D2O4T) Rabbit mAb 87809 20 µl
  • WB
  • IP
 H M 64,72 Rabbit IgG
IL-6 (D5W4V) XP® Rabbit mAb 12912 20 µl
  • WB
  • IP
  • IF
  • F
 M 24 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
 Rab Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
 M Horse 

Product Description

The Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides an economical means of detecting multiple components of the SASP in mouse samples. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit detects endogenous levels of its target protein. RANTES Antibody (Rodent Specific) detects transfected levels of rodent RANTES.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with recombinant human IL-1β protein, recombinant mouse TNF-α protein, recombinant mouse IL-6 protein, a synthetic peptide corresponding to residues surrounding Cys75 of mouse MCP-1 protein, or a synthetic peptide corresponding to residues surrounding Pro117 of human MMP-2 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of rodent RANTES protein or corresponding to residues surrounding Gly196 of murine PAI-1 protein. Polyclonal antibodies are purified by peptide affinity chromatography.

Background

Senescence is characterized by stable stress-induced proliferative arrest and resistance to mitogenic stimuli, as well as the secretion of proteins such as cytokines, growth factors, and proteases. These secreted proteins comprise the senescence-associated secretory phenotype (SASP). Senescent cells are thought to accumulate as an organism ages, and contribute to age-related diseases, including cancer, through promotion of inflammation and disruption of normal cellular function (1,2). The composition of the SASP varies, and SASP components can be either beneficial or deleterious in human disease, depending on the context (3).
The Mouse Reactive Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides a collection of antibodies to various SASP components, including TNF-α, interleukin-6 (IL-6), the multifunctional cytokine IL-1β, the chemokines RANTES/CCL5 and MCP-1, the matrix metalloprotease MMP-2, and the serine-protease inhibitor PAI-1.

Pathways

Explore pathways related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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