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8344
MRN Complex Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

MRN Complex Antibody Sampler Kit #8344

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MRN Complex Antibody Sampler Kit: Image 1

Immunohistochemical analysis of frozen SKOV-3 xenograft using Mre11 (31H4) Rabbit mAb.

MRN Complex Antibody Sampler Kit: Image 2

Western blot analysis of extracts from HeLa and HT-1080 cells, untreated or treated with UV, using Phospho-Mre11 (Ser676) Antibody (upper) or total Mre11 Antibody #4895 (lower).

MRN Complex Antibody Sampler Kit: Image 3

Western blot analysis of extracts from Jurkat and K562 cells, using RAD50 Antibody.

MRN Complex Antibody Sampler Kit: Image 4

Western blot analysis of extracts from Mv1Lu cells treated with UV or hydroxyurea (HU) for the indicated times, using Phospho-p95/NBS1 (Ser343) Antibody.

MRN Complex Antibody Sampler Kit: Image 5

Confocal immunofluorescent analysis of SK-MEL-28 (left) and GM07166 (right) cells using p95/NBS1 (D6J5I) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054.

MRN Complex Antibody Sampler Kit: Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

MRN Complex Antibody Sampler Kit: Image 7

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Mre11 (31H4) Rabbit mAb in the presence of control peptide (left) or Mre11 Blocking Peptide #1035 (right).

MRN Complex Antibody Sampler Kit: Image 8

Immunoprecipitation of p95/NBS1 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or p95/NBS1 (D6J5I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using p95/NBS1 (D6J5I) Rabbit mAb.

MRN Complex Antibody Sampler Kit: Image 9

Immunohistochemical analysis of paraffin-embedded lung carcinoma, using Mre11 (31H4) Rabbit mAb.

MRN Complex Antibody Sampler Kit: Image 10

Western blot analysis of extracts from various cell lines using p95/NBS1 (D6J5I) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

MRN Complex Antibody Sampler Kit: Image 11

Western blot analysis of extracts from HeLa and K562 cells, using Mre11 Rabbit (31H4) mAb.

To Purchase # 8344T
Product # Size Price
8344T
1 Kit  (5 x 20 µl) $ 391

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Mre11 (31H4) Rabbit mAb 4847 20 µl
  • WB
  • IP
  • IHC
H 81 Rabbit IgG
Phospho-Mre11 (Ser676) Antibody 4859 20 µl
  • WB
H 81 Rabbit 
Rad50 Antibody 3427 20 µl
  • WB
H Mk 153 Rabbit 
Phospho-p95/NBS1 (Ser343) Antibody 3001 20 µl
  • WB
H M Mi 95 Rabbit 
p95/NBS1 (D6J5I) Rabbit mAb 14956 20 µl
  • WB
  • IP
  • IF
H M R 95 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

MRN Complex Antibody Sampler Kit offers an economical way of detecting each target protein. The kit contains enough primary and secondary antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Antibodies detect endogenous levels of their respective proteins.

Source / Purification

Total polyclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to the amino terminus of human Rad50 or surrounding Ala740 of human p95/NBS1 protein. Activation state-specific polyclonal antibodies are produced by immunizing rabbits with synthetic phosphopeptides corresponding to residues surrounding Ser343 of human p95/NBS1 or Ser676 of human Mre11. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys496 of human Mre11A.

Background

The Mre11-Rad50-Nbs1 (MRN) complex is a key mediator of genome maintenance, playing important roles in meiosis, telomere stability at the ends of chromosomes, and the cellular responses to DNA damage (1-5). Homodimers of the Mre11 and Rad50 subunits form a tetramer core that binds directly to DNA and associates with the Nbs1 subunit (6). The complex functions as a sensor of DNA damage and localizes to DNA double-strand breaks. At these DNA lesions, the MRN complex tethers DNA ends and processes free strands via the endonuclease and exonuclease activities of Mre11. In addition to stimulating both homologous recombination and nonhomologous end joining repair DNA pathways, MRN activates DNA damage checkpoint signaling cascades regulating cell cycle progression. In some contexts, MRN is required for ATM activation and downstream phosphorylation of p53, BRCA1, and Chk2 (7). ATM also phosphorylates Mre11, Rad50, and Nbs1 (also known as p95 and Nibrin). Notably, Nbs1 Ser343 and Mre11 Ser676 are phosphorylated by ATM. Phosphorylation modulates function and association with many mediators, some of which include 53BP1, RPA, hSSB1, TRF2, BRCA1, FANCD2, CtP1, Histone H2AX, MDC1, and WRN helicase. Each subunit is essential for mammalian embryonic development, as mice with homozygous-null mutations in Mre11, Nbs1, or Rad50 are lethal. Furthermore, MRN complex function is required in developing lymphocytes for antigen receptor gene recombination initiated by the Rag-1 and Rag-2 recombinases. In humans, Mre11 and Nbs1 mutations cause chromosomal instability and radiosensitivity and are associated with ataxia-telangiectasia-like disorder (ATLD) and Nijmegen breakage syndrome (NBS), respectively (8). Genomic instability and cancer have been shown to develop in cells with genetic mutations within MRN complex genes.

  1. D'Amours, D. and Jackson, S.P. (2002) Nat Rev Mol Cell Biol 3, 317-27.
  2. van den Bosch, M. et al. (2003) EMBO Rep 4, 844-9.
  3. Ajimura, M. et al. (1993) Genetics 133, 51-66.
  4. Deng, Y. et al. (2009) Nature 460, 914-8.
  5. Lamarche, B.J. et al. (2010) FEBS Lett 584, 3682-95.
  6. Williams, R.S. et al. (2009) Cell 139, 87-99.
  7. Uziel, T. et al. (2003) EMBO J 22, 5612-21.
  8. Zhao, S. et al. (2000) Nature 405, 473-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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