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8344
MRN Complex Antibody Sampler Kit

MRN Complex Antibody Sampler Kit #8344

Western Blotting Image 1

Western blot analysis of extracts from HeLa and K562 cells, using Mre11 Rabbit (31H4) mAb.

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Western Blotting Image 2

Western blot analysis of extracts from HeLa and HT-1080 cells, untreated or treated with UV, using Phospho-Mre11 (Ser676) Antibody (upper) or total Mre11 Antibody #4895 (lower).

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Western Blotting Image 3

Western blot analysis of extracts from Jurkat and K562 cells, using RAD50 Antibody.

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Western Blotting Image 4

Western blot analysis of extracts from Mv1Lu cells treated with UV or hydroxyurea (HU) for the indicated times, using Phospho-p95/NBS1 (Ser343) Antibody.

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Western Blotting Image 5

Western blot analysis of extracts from various cell lines using p95/NBS1 (D6J5I) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

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Western Blotting Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IHC-P (paraffin) Image 7

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Mre11 (31H4) Rabbit mAb in the presence of control peptide (left) or Mre11 Blocking Peptide #1035 (right).

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IP Image 8

Immunoprecipitation of p95/NBS1 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or p95/NBS1 (D6J5I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using p95/NBS1 (D6J5I) Rabbit mAb.

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IHC-P (paraffin) Image 9

Immunohistochemical analysis of paraffin-embedded lung carcinoma, using Mre11 (31H4) Rabbit mAb.

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IF-IC Image 10

Confocal immunofluorescent analysis of SK-MEL-28 (left) and GM07166 (right) cells using p95/NBS1 (D6J5I) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054.

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IHC-F (frozen) Image 11

Immunohistochemical analysis of frozen SKOV-3 xenograft using Mre11 (31H4) Rabbit mAb.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Mre11 (31H4) Rabbit mAb 4847 20 µl
  • WB
  • IP
  • IHC
H 81 Rabbit IgG
Phospho-Mre11 (Ser676) Antibody 4859 20 µl
  • WB
H 81 Rabbit 
Rad50 Antibody 3427 20 µl
  • WB
H Mk 153 Rabbit 
Phospho-p95/NBS1 (Ser343) Antibody 3001 20 µl
  • WB
H M Mi 95 Rabbit 
p95/NBS1 (D6J5I) Rabbit mAb 14956 20 µl
  • WB
  • IP
  • IF
H M R 95 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

MRN Complex Antibody Sampler Kit offers an economical way of detecting each target protein. The kit contains enough primary and secondary antibody to perform two western blot experiments with each primary antibody.

Antibodies detect endogenous levels of their respective proteins.

Total polyclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to the amino terminus of human Rad50 or surrounding Ala740 of human p95/NBS1 protein. Activation state-specific polyclonal antibodies are produced by immunizing rabbits with synthetic phosphopeptides corresponding to residues surrounding Ser343 of human p95/NBS1 or Ser676 of human Mre11. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys496 of human Mre11A.

The Mre11-Rad50-Nbs1 (MRN) complex is a key mediator of genome maintenance, playing important roles in meiosis, telomere stability at the ends of chromosomes, and the cellular responses to DNA damage (1-5). Homodimers of the Mre11 and Rad50 subunits form a tetramer core that binds directly to DNA and associates with the Nbs1 subunit (6). The complex functions as a sensor of DNA damage and localizes to DNA double-strand breaks. At these DNA lesions, the MRN complex tethers DNA ends and processes free strands via the endonuclease and exonuclease activities of Mre11. In addition to stimulating both homologous recombination and nonhomologous end joining repair DNA pathways, MRN activates DNA damage checkpoint signaling cascades regulating cell cycle progression. In some contexts, MRN is required for ATM activation and downstream phosphorylation of p53, BRCA1, and Chk2 (7). ATM also phosphorylates Mre11, Rad50, and Nbs1 (also known as p95 and Nibrin). Notably, Nbs1 Ser343 and Mre11 Ser676 are phosphorylated by ATM. Phosphorylation modulates function and association with many mediators, some of which include 53BP1, RPA, hSSB1, TRF2, BRCA1, FANCD2, CtP1, Histone H2AX, MDC1, and WRN helicase. Each subunit is essential for mammalian embryonic development, as mice with homozygous-null mutations in Mre11, Nbs1, or Rad50 are lethal. Furthermore, MRN complex function is required in developing lymphocytes for antigen receptor gene recombination initiated by the Rag-1 and Rag-2 recombinases. In humans, Mre11 and Nbs1 mutations cause chromosomal instability and radiosensitivity and are associated with ataxia-telangiectasia-like disorder (ATLD) and Nijmegen breakage syndrome (NBS), respectively (8). Genomic instability and cancer have been shown to develop in cells with genetic mutations within MRN complex genes.

  1. D'Amours, D. and Jackson, S.P. (2002) Nat Rev Mol Cell Biol 3, 317-27.
  2. van den Bosch, M. et al. (2003) EMBO Rep 4, 844-9.
  3. Ajimura, M. et al. (1993) Genetics 133, 51-66.
  4. Deng, Y. et al. (2009) Nature 460, 914-8.
  5. Lamarche, B.J. et al. (2010) FEBS Lett 584, 3682-95.
  6. Zhao, S. et al. (2000) Nature 405, 473-7.
  7. Williams, R.S. et al. (2009) Cell 139, 87-99.
  8. Uziel, T. et al. (2003) EMBO J 22, 5612-21.
Entrez-Gene Id
4361 , 4683 , 10111
Swiss-Prot Acc.
P49959 , O60934 , Q92878
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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