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MTAP Antibody #4158

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  • WB

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 29
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MTAP Antibody detects endogenous levels of total MTAP protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the N terminus of human MTAP. Antibodies were purified by protein A and peptide affinity chromatography.

    Background

    MTAP is an enzyme that is essential for the salvage pathway for both adenine and methionine synthesis. MTAP catalyzes the cleavage of 5’-methylthioadenosine into adenine and 5-methylthio-D-ribose-1-phosphate. Adenine is then used to generate AMP whereas 5-methylthio-D-ribose-1-phosphate is converted into methionine (1,2). MTAP is expressed in all normal cells and tissues, although frequently lost in different human tumors, including pancreatic adenocarcinoma, neuroendocrine tumors, non-small cell lung carcinoma, and breast carcinoma. MTAP is usually codeleted with p16 (cdkN2a/ARF) (3-5). MTAP overexpression in breast cancer cells inhibits their ability to form colonies in soft agar, thereby implicating its function as a tumor suppressor (6).

    Alternate Names

    5'-methylthioadenosine phosphorylase; BDMF; c86fus; DMSFH; DMSMFH; epididymis luminal protein 249; epididymis secretory sperm binding protein; HEL-249; LGMBF; MeSAdo phosphorylase; methylthioadenosine phosphorylase; MSAP; MTA phosphorylase; MTAP; MTAPase; S-methyl-5'-thioadenosine phosphorylase

    For Research Use Only. Not for Use in Diagnostic Procedures.
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