Monoclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser15 of human p53, Thr180/Tyr182 of human p38 MAPK, Ser345 of human Chk1, Thr183/Tyr185 of human SAPK/JNK, Ser139 of human histone H2A.X, Tyr15 of human cdc2, or the carboxy terminus of human p21. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr68 of human Chk2. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
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