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Munc18-1 Antibody #8474

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Inquiry Info. # 8474

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    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 70
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Munc18-1 Antibody recognizes endogenous levels of total Munc18-1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near Asp453 of human Munc18-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Munc18-1 belongs to the Munc18 protein family that also includes Munc18-2 and Munc18-3. These proteins are highly conserved at the amino acid sequence level and play a critical role in membrane fusion. Munc18 proteins control SNARE complex formation (1,2) and interact with syntaxin (3). In Munc18-1 knockout mice, neurotransmitter release is completely abolished (4). In addition, Munc18-1 acts as a molecular chaperone for syntaxin-1, allowing for formation of the SNARE complex at the plasma membrane (5). Munc18-1 also acts as a regulator of vesicle docking during exocytosis (6).

    Alternate Names

    FLJ37475; MUNC18-1; N-Sec1; neuronal SEC1; NSEC1; p67; Protein unc-18 homolog 1; Protein unc-18 homolog A; RBSEC1; STXB1; STXBP1; syntaxin binding protein 1; Syntaxin-binding protein 1; Unc-18A; UNC18; Unc18-1; UNC18A

    For Research Use Only. Not for Use in Diagnostic Procedures.
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