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Munc18-1 (D4O6V) Rabbit Monoclonal Antibody (BSA and Azide Free) #76279

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  • WB

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #13414. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #13414

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    Munc18-1 (D4O6V) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total Munc18-1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr157 of human Munc18-1 protein.

    Background

    Munc18-1 belongs to the Munc18 protein family that also includes Munc18-2 and Munc18-3. These proteins are highly conserved at the amino acid sequence level and play a critical role in membrane fusion. Munc18 proteins control SNARE complex formation (1,2) and interact with syntaxin (3). In Munc18-1 knockout mice, neurotransmitter release is completely abolished (4). In addition, Munc18-1 acts as a molecular chaperone for syntaxin-1, allowing for formation of the SNARE complex at the plasma membrane (5). Munc18-1 also acts as a regulator of vesicle docking during exocytosis (6).

    Alternate Names

    FLJ37475; MUNC18-1; N-Sec1; neuronal SEC1; NSEC1; p67; Protein unc-18 homolog 1; Protein unc-18 homolog A; RBSEC1; STXB1; STXBP1; syntaxin binding protein 1; Syntaxin-binding protein 1; Unc-18A; UNC18; Unc18-1; UNC18A

    For Research Use Only. Not for Use in Diagnostic Procedures.
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