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Mutant Ras Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Mutant Ras Antibody Sampler Kit #18070

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Mutant Ras Antibody Sampler Kit: Image 1
Simple Western™ analysis of lysates (0.1 mg/mL) from THP-1 cells using Ras (G12D Mutant Specific) (D8H7) Rabbit mAb #14429. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Mutant Ras Antibody Sampler Kit: Image 2
Western blot analysis of extracts from various cell lines using Ras (G12V Mutant Specific) (D2H12) Rabbit mAb (upper), Ras (D2C1) Rabbit mAb #8955 (middle), and β-Actin (D6A8) #8457 (lower).
Mutant Ras Antibody Sampler Kit: Image 3
Western blot analysis of extracts from various cell lines using Ras (G12D Mutant Specific) (D8H7) Rabbit mAb (upper), Ras (D2C1) Rabbit mAb #8955 (middle), and β-Actin (D6A8) #8457 (lower).
Mutant Ras Antibody Sampler Kit: Image 4
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Mutant Ras Antibody Sampler Kit: Image 5
Western blot analysis of extracts from various cell lines using Ras (D2C1) Rabbit mAb.
To Purchase # 18070
Cat. # Size Qty. Price
1 Kit  (3 x 20 microliters)
$ 303

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Ras (G12V Mutant Specific) (D2H12) Rabbit mAb 14412 20 µl
  • WB
H 21 Rabbit IgG
Ras (G12D Mutant Specific) (D8H7) Rabbit mAb 14429 20 µl
  • WB
H 21 Rabbit IgG
Ras (D2C1) Rabbit mAb 8955 20 µl
  • WB
H M R Mk 21 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Rab Goat 

Kit Usage Information


Product Description

The Mutant Ras Antibody Sampler Kit provides an economical means of detecting common mutation in the Ras protein. The kit contains enough primary and secondary antibodies to perform at least two western blot experiments.

Specificity / Sensitivity

Each antibody in the Mutant Ras Antibody Sampler kit detects endogenous levels of its target protein. Ras (D2C1) Rabbit mAb recognizes endogenous levels of total Ras protein, specifically K-Ras and N-Ras. This antibody does not cross-react with H-Ras or R-Ras.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to G12V and G12D mutant sequences of human Ras protein. Ras (D2C1) Rabbit mAb was produced by immunizing rabbits with a recombinant protein specific to human K-Ras protein.


The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6). Additional oncogenic mutations of Ras have been observed at varying frequencies at codons 12, 13, and 61. The Gly12 to Val12 (G12V) mutation has been detected in a number of different cancers, including colorectal and thyroid cancer (7,8).
  1. Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-54.
  2. Avruch, J. et al. (1994) Trends Biochem Sci 19, 279-83.
  3. Buday, L. and Downward, J. (1993) Cell 73, 611-20.
  4. Huang, D.C. et al. (1993) Mol Cell Biol 13, 2420-31.
  5. Bos, J.L. (1989) Cancer Res 49, 4682-9.
  6. Ma, J. and Karplus, M. (1997) J Mol Biol 274, 114-31.
  7. Prior, I.A. et al. (2012) Cancer Res 72, 2457-67.
  8. Winder, T. et al. (2009) Oncol Rep 21, 1283-7.


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For Research Use Only. Not For Use In Diagnostic Procedures.
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