Render Target: STATIC
Render Timestamp: 2024-11-07T10:09:50.085Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-08-01 15:31:55.670
Product last modified at: 2024-11-01T12:00:32.746Z
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PDP - Template Name: Polyclonal Antibody (Epitope Tag)
PDP - Template ID: *******04464df

Myc-Tag Antibody #2272

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunofluorescence (Immunocytochemistry) 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Myc-Tag Antibody detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. The antibody may cross-react with c-myc protein.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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