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45893
PhosphoPlus® Myosin Light Chain 2 (Thr18/Ser19) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® Myosin Light Chain 2 (Thr18/Ser19) Antibody Duet #45893

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PhosphoPlus® Myosin Light Chain 2 (Thr18/Ser19) Antibody Duet: Image 1
Western blot analysis of extracts from various cell lines using Myosin Light Chain 2 (D18E2) Rabbit mAb.
PhosphoPlus® Myosin Light Chain 2 (Thr18/Ser19) Antibody Duet: Image 2
Western blot analysis of extracts from 293 and HeLa cells, vehicle-treated (-) or treated with the myosin kinase inhibitor ML-7 (75 uM, 30 min; +), using Phospho-Myosin Light Chain 2 (Thr18/Ser19) (E2J8F) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
PhosphoPlus® Myosin Light Chain 2 (Thr18/Ser19) Antibody Duet: Image 3
Western blot analysis of extracts from various cell lines using Phospho-Myosin Light Chain 2 (Thr18/Ser19) (E2J8F) Rabbit mAb (upper) or Myosin Light Chain 2 (D18E2) Rabbit mAb #8505 (lower). Prior to blocking, membranes were either untreated (-) or treated with Calf Intestinal Phosphatase (CIP) (250 units/ml, 2 hr; +) at 37ºC.
PhosphoPlus® Myosin Light Chain 2 (Thr18/Ser19) Antibody Duet: Image 4
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or post-processed with λ-phosphatase (right), using Phospho-Myosin Light Chain 2 (Thr18/Ser19) (E2J8F) Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
To Purchase # 45893
Cat. # Size Qty. Price
45893S
1 Kit
$ 550

Product Includes Quantity Reactivity MW(kDa) Isotype
Myosin Light Chain 2 (D18E2) Rabbit mAb 8505 100 µl H M R 18 Rabbit IgG
Phospho-Myosin Light Chain 2 (Thr18/Ser19) (E2J8F) Rabbit mAb 95777 100 µl H M R 18 Rabbit IgG

Kit Usage Information

Protocols

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).
  1. Ikebe, M. and Hartshorne, D.J. (1985) J. Biol. Chem. 260, 10027-10031.
  2. Tan, J. L. et al. (1992) Annu. Rev. Biochem. 61, 721-759.
  3. Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.
  4. Ikebe, M. et al. (2000) J. Biol. Chem. 262, 9569-9573.
  5. Satterwhite, L. L. et al. (1992) J. Cell Biol. 118, 595-605.
  6. Sanbe, A. et al. (1999) J. Biol. Chem. 274, 21085-21094.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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