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99935
Na,K-ATPase α1 (D4Y7E) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

Na,K-ATPase α1 (D4Y7E) Rabbit mAb (BSA and Azide Free) #99935

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: Na,K-ATPase α1 (D4Y7E) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from SK-MEL-28, SNB19 and A-204 cells using Na,K-ATPase α1 (D4Y7E) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Na,K-ATPase α1 (D4Y7E) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded SK-MEL-28 (left) and A-204 (right) cell pellets using Na,K-ATPase α1 (D4Y7E) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Na,K-ATPase α1 (D4Y7E) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human appendix using Na,K-ATPase α1 (D4Y7E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Na,K-ATPase α1 (D4Y7E) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Na,K-ATPase α1 (D4Y7E) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Na,K-ATPase α1 (D4Y7E) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of SK-MEL-28 (left), SNB19 (middle) or A-204 (right) cells using Na,K-ATPase α1 (D4Y7E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
To Purchase # 99935
Cat. # Size Qty. Price
99935SF
100 µg
$ 758

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 100
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #23565. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #23565

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Na,K-ATPase α1 (D4Y7E) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total Na,K-ATPase α1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro12 of human Na,K-ATPase α1 protein.

Background

The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit (reviewed in 1). Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the α subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).
  1. Therien, A.G. and Blostein, R. (2000) Am J Physiol Cell Physiol 279, C541-66.
  2. Féraille, E. et al. (1999) Mol Biol Cell 10, 2847-59.
  3. Fisone, G. et al. (1994) J Biol Chem 269, 9368-73.
  4. Feschenko, M.S. and Sweadner, K.J. (1995) J Biol Chem 270, 14072-7.
  5. Beguin, P. et al. (1994) J Biol Chem 269, 24437-45.
  6. Yingst, D.R. et al. (2004) Am J Physiol Renal Physiol 287, F713-21.
  7. Al-Khalili, L. et al. (2004) J Biol Chem 279, 25211-8.
  8. Tian, J. et al. (2006) Mol Biol Cell 17, 317-26.
  9. Liang, M. et al. (2006) J Biol Chem 281, 19709-19.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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