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Product last modified at: 2026-05-21T08:00:10.949Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
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Na,K-ATPase alpha1 (F7K1P) Feline Chimeric Monoclonal Antibody #24499

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  • IHC
  • IF

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Feline chimeric IgG1
    Application Key:
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as Na,K-ATPase alpha1 (F7K1P) Rabbit Monoclonal Antibody #58953.

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:50 - 1:200
    Immunofluorescence (Frozen) 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Na,K-ATPase alpha1 (F7K1P) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total Na,K-ATPase α1 protein. This antibody does not cross-react with other Na,K-ATPase α proteins. Species cross-reactivity for IF and IHC-P is mouse only.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    This recombinant chimeric antibody is engineered from Na,K-ATPase alpha1 (F7K1P) Rabbit Monoclonal Antibody #58953 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

    The parent antibody, Na,K-ATPase alpha1 (F7K1P) Rabbit Monoclonal Antibody #58953, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala498 of mouse Na,K-ATPase α1 protein.

    Background

    The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit (reviewed in 1). Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the α subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).

    Alternate Names

    AT1A1; Atp; Atp1a1; Atpa-1; ATPase, Na+/K+ transporting, alpha 1 polypeptide; BC010319; MGC38419; Na(+)/K(+) ATPase alpha-1 subunit; OTTMUSP00000017686; Sodium pump subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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