Render Target: STATIC
Render Timestamp: 2024-12-12T10:43:28.186Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:54:04.907
Product last modified at: 2024-10-09T14:15:13.254Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NAE1/APPBP1 (D9I4Z) Rabbit mAb #14321

Filter:
  • WB

    Supporting Data

    REACTIVITY
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NAE1/APPBP1 (D9I4Z) Rabbit mAb recognizes endogenous levels of total NAE1/APPBP1 protein. This antibody does not cross-react with E1 activating enzymes for either ubiquitin or other ubiquitin-like proteins.

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Bovine, Dog

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala179 of human NAE1/APPBP1 protein.

    Background

    Similar to ubiquitin, NEDD8 is covalently linked to target proteins through an enzymatic cascade composed of NEDD8-specific E1 (activating)- and E2 (conjugating)-enzymes (1,2). The E2 ligase specific for NEDD8 is Ubc12 (3-5). Ubc12 forms a heterodimeric conjugate with NEDD8 in order to catalyze the transfer of NEDD8 from E1 to lysine side chains of target proteins (1,2). Well known targets of NEDD8 are cullin-based RING E3 ligases. Neddylation of cullin isoforms activates the related ubiquitin E3 complex by promoting its interaction with a cognate ubiquitin-E2 ligase (6-7). Neddylation of Cul-1 complexes containing βTrCP and SKP2 has been shown to be required for controlling the stability of important signaling targets such as IκB, NF-κB, and p27 Kip (8-10), thereby regulating cell cycle progression, signaling cascades, and developmental programming processes (11).
    NAE1/APPBP1 (NEDD8-Activating Enzyme 1/Amyloid Beta Precursor Protein Binding Protein 1) exists in a heterodimeric complex with UBA3. This complex functions as an E1 NEDD8-activating enzyme, which utilizes ATP to adenylate the C-terminal glycine of NEDD8 (12-14). Research studies suggest that inhibition of the APPBP1-UBA3 complex may be of therapeutic value for the treatment of human cancers (15,16).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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