Render Target: STATIC
Render Timestamp: 2024-12-02T10:51:19.494Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-08-01 15:29:03.023
Product last modified at: 2024-11-19T21:30:16.330Z
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Naked1 Antibody #2262

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 59, 61
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Naked1 antibody detects endogenous levels of total Naked1 proteins.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln227 of human Naked1. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/β-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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