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NaPi2b/SLC34A2 Antibody #80309

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  • IP

Inquiry Info. # 80309

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 90-130
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NaPi2b/SLC34A2 Antibody recognizes endogenous levels of total NaPi-2b protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NaPi-2b protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The sodium-dependent phosphate transport protein 2B (NaPi-2b, SLC34A2) is a sodium dependent inorganic phosphate (Pi) transporter that regulates phosphate homeostasis in various organs, including the small intestine, lung, liver, and testis (1). In the small intestine, NaPi-2b localizes to the intestinal brush border membrane to mediate Pi reabsorption (2). In the lung, NaPi-2b is expressed in the apical membrane of type II alveolar cells and is involved in the synthesis of surfactant (3). Mutations in the corresponding SLC34A2 gene causes pulmonary alveolar microlithiasis, a rare autosomal recessive disorder characterized by the deposition of calcium phosphate microliths throughout the lungs (4). Research studies show aberrant expression of NaPi-2b in various type of cancer, including ovarian, breast, and lung cancer (5). Chromosomal rearrangements involving SLC34A2-ROS1 are seen in gastric carcinoma and non-small cell lung cancer and result in the formation of a SLC34A2-ROS1 chimeric protein that retains a constitutive kinase activity (6,7).

    Alternate Names

    FLJ90534; Na(+)-dependent phosphate cotransporter 2B; Na(+)/Pi cotransporter 2B; NaPi-2b; NAPI-3B; NAPI-IIb; NaPi3b; NPT2B; NPTIIb; PULAM; SLC34A2; Sodium-dependent phosphate transport protein 2B; Sodium-phosphate transport protein 2B; sodium-phosphate transporter; Sodium/phosphate cotransporter 2B; solute carrier family 34 (sodium phosphate), member 2; solute carrier family 34 (type II sodium/phosphate cotransporter), member 2; Solute carrier family 34 member 2; type II sodium-dependent phosphate transporter 3b

    For Research Use Only. Not for Use in Diagnostic Procedures.
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