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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
RIP (D94C12) XP® Rabbit mAb 3493 20 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H M R Hm Mk 78 Rabbit IgG
Phospho-RIP (Ser166) (D1L3S) Rabbit mAb 65746 20 µl
Western Blotting
H 78-82 Rabbit IgG
MLKL (D2I6N) Rabbit mAb 14993 20 µl
Western Blotting
H 54 Rabbit IgG
Phospho-MLKL (Ser358) (D6H3V) Rabbit mAb 91689 20 µl
Western Blotting
H 54 Rabbit IgG
RIP3 (E1Z1D) Rabbit mAb 13526 20 µl
Western Blotting Immunoprecipitation
H 46-62 Rabbit IgG
Phospho-RIP3 (Ser227) (D6W2T) Rabbit mAb 93654 20 µl
Western Blotting Immunofluorescence
H 46-62 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The Necroptosis Antibody Sampler Kit provides an economical means of detecting total and phosphorylated proteins associated with necroptosis. The kit includes enough antibody to perform two western blots with each primary antibody.

Specificity / Sensitivity

Each antibody in the Necroptosis Antibody Sampler Kit detects endogenous levels of its target protein. MLKL (D2I6N) Rabbit mAb cross-reacts with an unidentified band at 130 kDa in some cell lines. Phospho-MLKL (Ser358) (D6H3V) Rabbit mAb may also bind to MLKL when dually phosphorylated at Thr357 and Ser358.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Leu190 of human RIP, residues near the carboxyl terminus of human RIP3, and residues near the carboxyl terminus of human MLKL. Phospho-specific monoclonal antibodies are produced by immunizing rabbits with synthetic phospho-peptides corresponding to Ser166 of human RIP, Ser227 of human RIP3, and Ser358 of human MLKL.

Necroptosis, a regulated pathway for necrotic cell death, is triggered by a number of inflammatory signals, including cytokines in the tumor necrosis factor (TNF) family, pathogen sensors such as toll-like receptors (TLRs), ischemic injury, and neurodegenerative diseases (1-3). The process is negatively regulated by caspases and is initiated through a complex containing the RIP and RIP3 kinases, typically referred to as the necrosome. Necroptosis is inhibited by a small molecule inhibitor of RIP, necrostatin-1 (Nec-1) (4). RIP is phosphorylated at several sites within the kinase domain that are sensitive to Nec-1, including Ser14, Ser15, Ser161, and Ser166 (5). During necroptosis, RIP3 is phosphorylated at Ser227, leading to recruitment and phosphorylation of MLKL at Thr357 and Ser358 (6). Phosphorylation of MLKL results in its oligomerization and translocation to the plasma membrane, where it effects membrane integrity (7-10).

1.  Christofferson, D.E. and Yuan, J. (2010) Curr Opin Cell Biol 22, 263-8.

2.  Kaczmarek, A. et al. (2013) Immunity 38, 209-23.

3.  Zhou, W. and Yuan, J. (2014) Semin Cell Dev Biol 35, 14-23.

4.  Degterev, A. et al. (2008) Nat Chem Biol 4, 313-21.

5.  Ofengeim, D. and Yuan, J. (2013) Nat Rev Mol Cell Biol 14, 727-36.

6.  Sun, L. et al. (2012) Cell 148, 213-27.

7.  Cai, Z. et al. (2014) Nat Cell Biol 16, 55-65.

8.  Chen, X. et al. (2014) Cell Res 24, 105-21.

9.  Wang, H. et al. (2014) Mol Cell 54, 133-46.

10.  Dondelinger, Y. et al. (2014) Cell Rep 7, 971-81.

Entrez-Gene Id 197259 , 8737 , 11035
Swiss-Prot Acc. Q8NB16 , Q13546 , Q9Y572

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

Necroptosis Antibody Sampler Kit