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Nectin-1/PVRL1 (F7J9O) Rabbit Monoclonal Antibody #28147

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Nectin-1/PVRL1 (F7J9O) Rabbit Monoclonal Antibody recognizes endogenous levels of total nectin-1/PVRL1 protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln478 of human nectin-1/PVRL1 protein.

    Background

    Nectin cell adhesion molecule 1 (Nectin-1), also known as poliovirus receptor-related 1 (PVRL1) and CD111, is a single-pass type I membrane glycoprotein and is part of the immunoglobulin (Ig) superfamily of cell adhesion proteins (1). Nectins are composed of an ectodomain containing three tandem Ig-like regions arranged as an N-terminal variable-like (V) domain followed by two constant-like (C) domains, a single transmembrane region, and a cytoplasmic domain that binds to the actin cytoskeleton through the adaptor protein afadin (2). Nectin-1 plays important roles in adherens junction (AJ) formation in epithelial cells and neuronal synapses (1,3). Like other nectin family members, nectin-1 is used as an entry receptor for several mammalian viruses, including herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), porcine pseudorabies virus (PRV), bovine herpes virus type 1 (BHV-1), and simian B virus (4-6). Nectin-1 also plays a role in the immune system, where it interacts with CD96 expressed on natural killer (NK) cells and T cell subsets, and modulates cancer cell and virally infected cell clearance (7,8).   

    Alternate Names

    CD111; CLPED1; ectodermal dysplasia 4 (Margarita Island type); ED4; herpes simplex virus type 1 sensitivity; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; HIgR; HV1S; HVEC; MGC142031; MGC16207; NECT1; nectin 1; Nectin cell adhesion molecule 1; Nectin-1; NECTIN1; OFC7; poliovirus receptor-like 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); Poliovirus receptor-related protein 1; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12

    For Research Use Only. Not for Use in Diagnostic Procedures.
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