Render Target: STATIC
Render Timestamp: 2024-12-12T11:07:35.719Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-08-01 15:30:26.045
Product last modified at: 2024-12-05T14:45:13.227Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

NEK7 Antibody #10054

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 32
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NEK7 Antibody recognizes endogenous levels of total NEK7 protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu22 of human NEK7 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    NEK7 (NIMA-related protein kinase 7), is one of a family of serine/threonine kinases, named NEK1–NEK11, which are involved in the control of mitosis and other non-mitotic functions (1-6). NEK7 is the smallest NIMA-related kinase in mammals, and is widely expressed by many tissues (1-3). Its role in mitosis relates to centrosome enrichment and microtubule nucleation, and similar to other NIMA-related kinases, NEK7 is localized to the microtubule-organizing center (MTOC) at the time of mitotic entry (3,6). NEK7 has also been demonstrated to be essential for NLRP3 inflammasome activation by directly binding to the leucine-rich repeat (LRR) domain of NLRP3 (4-6).
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