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9781
Neurofilament Antibody Sampler Kit

Neurofilament Antibody Sampler Kit #9781

Western Blotting Image 1

Western blot analysis of extracts from rat brain, untreated or treated with lambda phosphatase, using Neurofilament-M (RMO 14.9) Mouse mAb.

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Western Blotting Image 2

Western blot analysis of extracts from rat brain, mouse brain, U87-MG cells, C6 cells and human cerebellum, using Neurofilament-L (DA2) Mouse mAb.

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Western Blotting Image 3

Western blot analysis of extracts from mouse brain, HeLa cells and rat brain, using Neurofilament-L (C28E10) Rabbit mAb.

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Western Blotting Image 4

Western blot analysis of extracts from mouse brain, rat brain or CAD cells, using Neurofilament-H (RMdO 20) Mouse mAb.

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Western Blotting Image 5

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IHC-P (paraffin) Image 6

Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-M (RMO 14.9) Mouse mAb.

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IHC-P (paraffin) Image 7

Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (DA2) Mouse mAb.

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IHC-P (paraffin) Image 8

Immunohistochemical analysis of paraffin-embedded mouse brain using Neurofilament-L (C28E10) Rabbit mAb.

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IHC-P (paraffin) Image 9

Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-H (RMdO 20) Mouse mAb.

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IF-F Image 10

Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-M (RMO 14.9) Mouse mAb (green) and Calbindin Antibody #2136 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded human medulloblastoma, using Neurofilament-L (DA2) Mouse mAb.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (C28E10) Rabbit mAb in the presence of control peptide (left) or Neurofilament-L blocking peptide #1005 (right).

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IF-F Image 13

Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-H (RMdO 20) Mouse mAb (green) and Calbindin Antibody #2136 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

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IF-F Image 14

Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-L (DA2) Mouse mAb (green) and CREB (48H2) Rabbit mAb #9197 (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

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IF-F Image 15

Confocal immunofluorescent analysis of normal rat cerebellum using Neurofilament-L (C28E10) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Neurofilament-M (RMO 14.9) Mouse mAb 2838 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R 160 Mouse IgG1
Neurofilament-L (DA2) Mouse mAb 2835 20 µl
  • WB
  • IHC
  • IF
H M R 70 Mouse IgG1
Neurofilament-L (C28E10) Rabbit mAb 2837 20 µl
  • WB
  • IHC
  • IF
H M R 70 Rabbit IgG
Neurofilament-H (RMdO 20) Mouse mAb 2836 20 µl
  • WB
  • IHC
  • IF
H M R 180-220 Mouse IgG1
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

The Neurofilament Antibody Sampler Kit provides an economical means of evaluating neurofilaments. The kit contains enough primary and secondary antibodies to perform two western blot experiments per primary antibody.

Neurofilament-L (DA2) Mouse mAb and Neurofilament-L (C28E10) Rabbit mAb detect endogenous levels of total Neurofilament-L protein. Neurofilament-M (RMO 14.9) Mouse mAb detects endogenous levels of total Neurofilament-M protein. Neurofilament-H (RMdO 20) Mouse mAb detects endogenous levels of total Neurofilament-H protein. Neurofilament-H (RMdO 20) Mouse mAb has been reported to detect NFM and NFH in human samples but only NFH in mouse, rat or bovine samples (Lee, V.M. et al., 1988).

Monoclonal antibodies are produced by immunizing animals with purified and enzymatically dephosphorylated pig neurofilament, light chain (Neurofilament-L (DA2)), a synthetic peptide surrounding Glu450 of human Neurofilament-L (Neurofilament-L (C28E10)), rat neurofilament medium chain

(Neurofilament-M (RMO 14.9)), or rat neurofilament (Neurofilament-H (RMdO 20)).

#2836 Monoclonal antibody is produced by immunizing animals with rat neurofilament.

#2835 Monoclonal antibody is produced by immunizing animals with purified and enzymatically dephosphorylated pig neurofilament, light chain.

#2837 Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Glu450 of human Neurofilament-L.

#2838 Monoclonal antibody is produced by immunizing animals with rat neurofilament, medium chain.

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).

  1. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-55.
  2. Cohlberg, J.A. et al. (1995) J Biol Chem 270, 9334-9.
  3. Hisanaga, S. et al. (1994) Mol Biol Cell 5, 161-72.
  4. Sihag, R.K. et al. (1999) J Neurochem 72, 491-9.
Entrez-Gene Id
4744 , 4741 , 4747
Swiss-Prot Acc.
P12036 , P07197 , P07196
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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