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60044
Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free) #60044

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free)
Western blot analysis of extracts from various tissues using Neurofilament-M (E7Y8W) Mouse mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Negative expression of Neurofilament-M protein in mouse pancreas tissue is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human basal ganglia using Neurofilament-M (E7Y8W) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human caudate nucleus using Neurofilament-M (E7Y8W) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human basal ganglia using Neurofilament-M (E7Y8W) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded peripheral nerve within human prostate adenocarcinoma using Neurofilament-M (E7Y8W) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human heart using Neurofilament-M (E7Y8W) Mouse mAb. Note staining of peripheral nerve. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human brain using Neurofilament-M (E7Y8W) Mouse mAb (left) compared to concentration-matched Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded A-673 cell pellet (left, positive) or T24 cell pellet (right, negative) using Neurofilament-M (E7Y8W) Mouse mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse hippocampus labeled with Neurofilament-M (E7Y8W) Mouse mAb (left, green) and co-labeled with Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (right, cyan pseudocolor), GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (right, red), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of A-673 cells (left, positive) and T24 cells (right, negative) using Neurofilament-M (E7Y8W) Mouse mAb (green), DyLight 650 Phalloidin #12956 (red), and DAPI #4083 (blue). Data were generated using the standard formulation of this product.
To Purchase # 60044
Cat. # Size Qty. Price
60044SF
100 µg
$ 758

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 160
Source/Isotype Mouse IgG2a kappa

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #53086. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #53086

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Neurofilament-M (E7Y8W) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total Neurofilament-M protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Neurofilament-M protein.

Background

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).
  1. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-55.
  2. Cohlberg, J.A. et al. (1995) J Biol Chem 270, 9334-9.
  3. Hisanaga, S. et al. (1994) Mol Biol Cell 5, 161-72.
  4. Sihag, R.K. et al. (1999) J Neurochem 72, 491-9.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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