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24544
Neuronal Marker IF Antibody Sampler Kit II

Neuronal Marker IF Antibody Sampler Kit II #24544

IF-F Image 1

Confocal immunofluorescent analysis of Doublecortin (A8L1U) Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red) staining in the subventricular zone of adult mouse brain. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Western Blotting Image 2

Western blot analysis of extracts from mouse brain, C2C12 cells, and rat brain using NeuN (D4G4O) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 3

Western blot analysis of extracts from mouse and rat brain, using GFAP (GA5) Mouse mAb.

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Western Blotting Image 4

Western blot analysis of extracts from mouse brain and C2C12 cells using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Flow Cytometry Image 5

Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

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IF-F Image 6

Confocal immunofluorescent analysis of P5 mouse cerebellum using Doublecortin (A8L1U) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 7

Immunohistochemical analysis of paraffin-embedded mouse cerebellum using NeuN (D4G4O) XP® Rabbit mAb.

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IHC-P (paraffin) Image 8

Immunohistochemical analysis of paraffin-embedded human medulloblastoma, using GFAP (GA5) Mouse mAb.

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IHC-P (paraffin) Image 9

Immunohistochemical analysis of a paraffin-embedded human Alzheimer's brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb.

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IF-IC Image 10

Confocal immunofluorescent analysis of HeLa cells using Ki-67 (D3B5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded mouse colon (myenteric plexus) using NeuN (D4G4O) XP® Rabbit mAb.

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IF-IC Image 12

Confocal immunofluorescent analysis of SNB19 (positive, left) and HeLa (negative, right) cells, using GFAP (GA5) Mouse mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 13

Immunohistochemical analysis of a paraffin-embedded mouse brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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IF-F Image 14

Confocal immunofluorescent analysis of the ventricular zone in P21 mouse brain using Ki-67 (D3B5) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 15

Immunohistochemical analysis of paraffin-embedded human glioblastoma multiform using NeuN (D4G4O) XP® Rabbit mAb.

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IF-F Image 16

Confocal immunofluorescence image of rat hippocampus labeled with GFAP (GA5) Mouse mAb (red), Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green), and CREB (48H2) Rabbit mAb #9197 (blue).

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IF-IC Image 17

Confocal immunofluorescent analysis of mouse primary neurons and neuroglia using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IF-F Image 18

Confocal immunofluorescent analysis of mouse hippocampus (left), cortex (middle), and cerebellum (right) using NeuN (D4G4O) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IF-F Image 19

Confocal immunofluorescent analysis of rat brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green) and Synaptophysin (7H12) Mouse mAb (IF Formulated) #9020 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Doublecortin (A8L1U) Rabbit mAb 14802 20 µl
  • IF
M 45 Rabbit IgG
NeuN (D4G4O) XP® Rabbit mAb 24307 20 µl
  • WB
  • IHC
  • IF
H M R 46-55 Rabbit IgG
GFAP (GA5) Mouse mAb 3670 20 µl
  • WB
  • IHC
  • IF
H M R 50 Mouse IgG1
Myelin Basic Protein (D8X4Q) XP® Rabbit mAb 78896 20 µl
  • WB
  • IHC
  • IF
H M R 12-18 Rabbit IgG
Ki-67 (D3B5) Rabbit mAb 9129 20 µl
  • IF
  • F
H M R 359 Rabbit IgG

The Neuronal Marker IF Antibody Sampler Kit II provides an economical means for labeling cell types and cell structures by immunofluorescence (IF-F).

Each antibody in the Neuronal Marker IF Antibody Sampler Kit II has been validated for IF-F, recognizes only its specific target, and does not cross-react with other family members. Doublecortin (A8L1U) Rabbit mAb recognizes endogenous levels of total doublecortin protein. NeuN (D4G4O) XP® Rabbit mAb recognizes endogenous levels of total NeuN protein. GFAP (GA5) Mouse mAb detects endogenous levels of total GFAP protein. Myelin Basic Protein (D8X4Q) XP® Rabbit mAb recognizes endogenous levels of total myelin basic protein. Ki-67 (D3B5) Rabbit mAb recognizes endogenous levels of total Ki-67 protein.

Rabbit and Mouse monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human doublecortin protein, or with recombinant protein specific to the amino terminus of human NeuN protein, or with native GFAP purified from pig spinal cord, or with a synthetic peptide corresponding to residues surrounding Ala185 of human myelin basic protein, or with a synthetic peptide corresponding to residues near the amino terminus of human Ki-67 protein.

The antibodies in this kit serve as markers to determine cell types in the brain. Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. Doublecortin is expressed in neuronal precursor cells and expression is downregulated as neurons mature (1). Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (2). As Doublecortin is downregulated, NeuN is upregulated (1,2). GFAP filaments are characteristic of differentiated and mature brain astrocytes. In addition, GFAP is commonly used by investigators as a marker for intracranial and intraspinal tumors arising from astrocytes (3). Myelin basic protein (MBP) is an abundant central nervous system (CNS) myelin membrane protein that plays an important role in nerve myelination. Myelin sheaths are multi-layered membranes derived from oligodendrocytes (4). Ki-67 is universally expressed among proliferating cells and absent in quiescent cells. Specifically, it is detected in proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase (5).

  1. Brown, J.P. et al. (2003) J Comp Neurol 467, 1-10.
  2. Mullen, R.J. et al. (1992) Development 116, 201-11.
  3. Harauz, G. and Boggs, J.M. (2013) J Neurochem 125, 334-61.
  4. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
  5. Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93.
Entrez-Gene Id
1641 , 2670 , 4288 , 4155 , 4440 , 146713
Swiss-Prot Acc.
O43602 , P14136 , P46013 , P02686 , O43347 , A6NFN3
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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