| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 24544T | 1 Kit (5 x 20 microliters) |
|
| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| Doublecortin (A8L1U) Rabbit mAb 14802 | 20 µl |
|
M | Rabbit IgG | |
| NeuN (D4G4O) XP® Rabbit mAb 24307 | 20 µl |
|
H M R | 46-55 | Rabbit IgG |
| GFAP (GA5) Mouse mAb 3670 | 20 µl |
|
H M R | 50 | Mouse IgG1 |
| Myelin Basic Protein (D8X4Q) XP® Rabbit mAb 78896 | 20 µl |
|
H M R | 12-18 | Rabbit IgG |
| Ki-67 (D3B5) Rabbit mAb 9129 | 20 µl |
|
H M R | Rabbit IgG |
Product Information
Rabbit and Mouse monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human doublecortin protein, or with recombinant protein specific to the amino terminus of human NeuN protein, or with native GFAP purified from pig spinal cord, or with a synthetic peptide corresponding to residues surrounding Ala185 of human myelin basic protein, or with a synthetic peptide corresponding to residues near the amino terminus of human Ki-67 protein.
The antibodies in this kit serve as markers to determine cell types in the brain. Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. Doublecortin is expressed in neuronal precursor cells and expression is downregulated as neurons mature (1). Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (2). As Doublecortin is downregulated, NeuN is upregulated (1,2). GFAP filaments are characteristic of differentiated and mature brain astrocytes. In addition, GFAP is commonly used by investigators as a marker for intracranial and intraspinal tumors arising from astrocytes (3). Myelin basic protein (MBP) is an abundant central nervous system (CNS) myelin membrane protein that plays an important role in nerve myelination. Myelin sheaths are multi-layered membranes derived from oligodendrocytes (4). Ki-67 is universally expressed among proliferating cells and absent in quiescent cells. Specifically, it is detected in proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase (5).
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