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Commit: ab362d7804f80c42c0e61931a854c3f7873f3526
XML generation date: 2026-06-10 03:28:47.537
Product last modified at: 2026-06-10T08:00:16.978Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Neuropeptide Y (D7Y5A) Horse Chimeric Monoclonal Antibody #32795

Filter:
  • IF

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Horse chimeric IgG4
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as Neuropeptide Y (D7Y5A) Rabbit Monoclonal Antibody #11976.

    Product Usage Information

    Application Dilution
    Immunofluorescence (Frozen) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Neuropeptide Y (D7Y5A) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of precursor and mature neuropeptide Y. This antibody does not cross-react with peptide YY or peptide YY (3-36).

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    This recombinant chimeric antibody is engineered from Neuropeptide Y (D7Y5A) Rabbit Monoclonal Antibody #11976 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

    The parent antibody, Neuropeptide Y (D7Y5A) Rabbit Monoclonal Antibody #11976, is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human mature neuropeptide Y.

    Background

    Neuropeptide Y (NPY) is a 36 amino acid peptide that belongs to the pancreatic polypeptide (PP) family, which also includes peptide YY (PYY) (1). The mature 36-residue NPY is produced from a larger pre-pro 97-residue NPY precursor through a series of cleavage reactions at dibasic sites and C-terminal amidation of the peptide product (2). NPY is widely expressed in the central nervous system (3) and exerts its action through stimulation of 5 different receptors (Y1-Y5) that belong to the G protein-coupled receptor family (4). NPY in the hypothalamus exhibits orexigenic activity through activation of Y1 and Y5 receptors (5). NPY is involved in the control of bone homeostasis, through the regulation of osteoblast activity by Y1 and Y2 receptors (6), and the regulation of testosterone secretion by activating Y1 receptor in testicular vessels (7). Research studies suggest that modulation of NPY activity and signaling represents a potential strategy for the development of appetite control and antiobesity agents (8).

    Alternate Names

    C-flanking peptide of NPY; CPON; Neuropeptide tyrosine; Neuropeptide Y; NPY; prepro-neuropeptide Y; Pro-neuropeptide Y; PYY4

    For Research Use Only. Not for Use in Diagnostic Procedures.
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