Render Target: STATIC
Render Timestamp: 2024-10-09T10:10:01.166Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Neuropilin-2 Antibody #89189

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 120, 130
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Neuropilin-2 Antibody recognizes endogenous levels of total neuropilin-2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro681 of human neuropilin-2 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single-pass, transmembrane signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).

    Neuropilin-2, a single-pass, transmembrane glycoprotein, can also bind to VEGF165 and VEGF145, but not VEGF121. Thus, in addition to neuronal guidance, neuropilin-2 plays a role in angiogenesis and cardiovascular functions (4). Although neuropilin-2 has been linked to tumor biology (e.g., pancreatic cancer, bladder cancer, lung cancer, and neuroblastoma), its role in tumor pathogenesis is still under investigation (4,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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