Render Target: STATIC
Render Timestamp: 2025-03-19T11:30:12.599Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:06:14.401
Product last modified at: 2025-01-05T15:45:07.849Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

NFI-C Antibody #11911

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 55-65
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NFI-C Antibody recognizes endogenous levels of total NFI-C protein. This antibody also recognizes overexpressed NFI-B protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala263 of human NFI-C protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    NFI-C belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). NFI-C is expressed in various tissues and regulates TGF-β dependent tooth development and hair follicle cycling (3-5). Research studies have shown that NFI-C directly represses FoxF1 transcription and suppresses the motility and invasiveness of breast cancer cells (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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