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Nipah/Hendra virus Nucleocapsid (F5N9K) Rabbit Monoclonal Antibody

Nipah/Hendra virus Nucleocapsid (F5N9K) Rabbit Monoclonal Antibody #32952

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Product Specifications

REACTIVITY	Vir
SENSITIVITY	Endogenous (IHC-P), Transfected (W)
MW (kDa)	65
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

Vir-Virus

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:400 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Nipah/Hendra virus Nucleocapsid (F5N9K) Rabbit Monoclonal Antibody recognizes endogenous levels of total Nipah/Hendra virus nucleocapsid protein. This antibody has been shown to recognize both Malaysia and Bangladesh strains of Nipah virus.

Species Reactivity:

Virus

Source / Purification

Nipah/Hendra virus Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala530 of Nipah/Hendra virus nucleocapsid protein.

Background

Nipah virus (NiV) and Hendra virus (HeV) are highly pathogenic zoonotic paramyxoviruses belonging to the Henipavirus genus. They both possess a non-segmented, negative-sense single-stranded RNA genome, which is tightly encapsidated by the nucleocapsid (N) protein to form a helical ribonucleoprotein (RNP) complex, the fundamental unit for viral RNA synthesis and genome protection (1). The N protein, which is largely conserved between the two viruses, is a major structural component and a key player in its replication cycle. The primary function of the NiV/HeV N protein is to encapsidate viral genomic and antigenomic RNA. Encapsidation is essential for genome protection, template assembly for RNA synthesis, and nucleocapsid assembly. It also interacts with other viral proteins, such as the viral phosphoprotein (P), which acts as a chaperone for newly synthesized N protein subunits (2). Understanding the structure and function of the NiV and HeV N protein is critical for developing targeted antiviral therapies against this and other highly pathogenic viruses in the Paramyxoviridae family (3).

Alternate Names

Hendra henipavirus N protein; nucleocapsid

Database Links

UniProt ID: O89339

Entrez-Gene Id: 1446470

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