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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Non-phospho PTEN (Ser380/Thr382/Thr383) Antibody detects endogenous levels of PTEN only when dephosphorylated at Ser380, Thr382 and Thr383.

Species predicted to react based on 100% sequence homology: Human, Chicken

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser380/Thr382/Thr383 of human PTEN. Antibodies are purified by peptide affinity chromatography.

PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).

1.  Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.

2.  Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.

3.  Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.

4.  Wan X and Helman LJ (2003) Oncogene 22, 8205–11

5.  Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.

6.  Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.

7.  Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.

8.  Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.

9.  Funamoto, S. et al. (2002) Cell 109, 611-23.

10.  Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

Entrez-Gene Id 5728
Swiss-Prot Acc. P60484

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Non-phospho PTEN (Ser380/Thr382/Thr383) Antibody