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Normal Rabbit IgG #2729

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  • IP
  • ChIP
  • C&T

    Supporting Data

    Concentration 1 mg/ml
    SENSITIVITY Endogenous
    Source/Isotype Rabbit
    Application Key:
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    • C&T-CUT & Tag 

    Product Information

    Product Description

    Normal Rabbit IgG is an unconjugated rabbit polyclonal antibody that is routinely used as a non-specific IgG control in chromatin immunoprecipitation using our SimpleChIP® Enzymatic Chromatin IP Kits #9002 and #9003.

    Product Usage Information

    Important! Dilute this control antibody to the same concentration (not dilution) as the specific test antibody in the chromatin immunoprecipitation. This is typically 1 μl (1 μg) to 5 μl (5 μg) of control antibody for one immunoprecipitation. Higher background signal may result if an excessive amount of rabbit IgG isotype control is used.

    Application Dilution
    CUT&Tag 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Source / Purification

    The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and prepared by Protein A chromatography.

    Background

    Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

      Alternate Names

      chip; chip-on-chip; chip-validated; chipkit; chromatin ip; immunoprecipitation; isotype control

      For Research Use Only. Not for Use in Diagnostic Procedures.
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