Render Target: STATIC
Render Timestamp: 2024-10-09T10:09:41.304Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NOTUM (F1E9C) Rabbit mAb #92654

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 55,62
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NOTUM (F1E9C) Rabbit mAb recognizes endogenous levels of total NOTUM protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His422 of human NOTUM protein.

    Background

    NOTUM is an extracellular carboxylesterase and a key negative regulator of the Wnt signaling pathway. The palmitoleoylation of Wnt is required for its binding to Frizzled receptors, which is essential for Wnt signaling activation. NOTUM removes the palmitoleate moiety on Wnt ligands to inactivate Wnt signaling (1,2). The function of NOTUM is dependent on glypicans, which bind both NOTUM and Wnt to co-localize them at the cell surface. NOTUM is expressed in the liver (3), bone (4), epithelial cells in the intestine (5), and brain (6) to regulate tissue formation and function. Biological aging may result from increased NOTUM expression, while inhibition of NOTUM may rejuvenate stem cells to promote tissue regeneration (5). In colorectal cancer and gastric cancer, aberrant high NOTUM expression promotes proliferation and metastasis. Knockdown or inhibition of NOTUM activity suppresses its cancer-promoting effects (7-9). Inhibition of NOTUM activity may represent a new approach to treat disease where aberrant NOTUM activity has been identified as the underlying cause (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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