NSUN2 (E9Y5E) Rabbit Monoclonal Antibody #52901
- WB
- IHC
- IF
Product Specifications
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Frozen) | 1:1600 - 1:3200 |
| Immunofluorescence (Immunocytochemistry) | 1:3200 - 1:12800 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
NSUN2 is an 86 kDa member of the NSUN family that is predominantly localized to the nucleus and methylates a variety of tRNAs and other RNA substrates (3). NSUN2 has been found to play a role in the methylation of pre-tRNALeu, tRNAGly, tRNAVal, tRNALeu, and tRNAAsp (1,4,5). It has also been found to methylate mRNAs and several ncRNAs, such as vtRNAs (6-8). Elevated NSUN2 protein levels have been reported in multiple types of human cancers, including breast, prostate, kidney, bladder, and liver, among others (9). The methyltransferase activity of NSUN2 is reduced upon Aurora B kinase-mediated phosphorylation at Ser139 (10), and there is potential for NSUN2 to be used as a therapeutic target or diagnostic marker for Aurora B linked cancers (9).
- Trixl, L. and Lusser, A. (2019) Wiley Interdiscip Rev RNA 10, e1510.
- Bohnsack, K.E. et al. (2019) Genes (Basel) 10, pii: E102. doi: 10.3390/genes10020102.
- Bourgeois, G. et al. (2015) PLoS One 10, e0133321.
- Brzezicha, B. et al. (2006) Nucleic Acids Res 34, 6034-43.
- Tuorto, F. et al. (2012) Nat Struct Mol Biol 19, 900-5.
- Tang, H. et al. (2015) Aging (Albany NY) 7, 1143-58.
- Xing, J. et al. (2015) Mol Cell Biol 35, 4043-52.
- Hussain, S. et al. (2013) Cell Rep 4, 255-61.
- Okamoto, M. et al. (2012) DNA Cell Biol 31, 660-71.
- Sakita-Suto, S. et al. (2007) Mol Biol Cell 18, 1107-17.
Alternate Names
5-methycytoisine methyltransferase; cDNA FLJ76184, highly similar to Homo sapiens NOL1/NOP2/Sun domain family, member 2 (NSUN2), mRNA; FLJ20303; hTrm4; MISU; mRNA cytosine C(5)-methyltransferase; MRT5; Myc-induced SUN domain-containing protein; Myc-induced SUN-domain-containing protein; NOL1/NOP2/Sun domain family 2; NOL1/NOP2/Sun domain family member 2; NOL1/NOP2/Sun domain family, member 2; NOP2/Sun domain family, member 2; NOP2/Sun RNA methyltransferase 2; NOP2/Sun RNA methyltransferase family member 2; NSUN2; RNA cytosine C(5)-methyltransferase NSUN2; SAKI; substrate of AIM1/Aurora kinase B; TRM4; tRNA (cytosine-5-)-methyltransferase NSUN2; tRNA (cytosine(34)-C(5))-methyltransferase; tRNA cytosine C(5)-methyltransferase; tRNA methyltransferase 4 homolog
Limited Uses
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