NTF2 (5A3) Mouse mAb #3053
- WB
- IF
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 14 |
| Source/Isotype | Mouse IgG2a |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Nuclear transport factor 2 (NTF2) regulates the subcellular distribution and function of Ran (4-5). The NTF2 homodimer facilitates the diffusion of RanGDP through NPCs via transient interactions with phenylalanine-glycine (FG) repeat domains on NPC proteins. NTF2 stabilizes the GDP-bound form of Ran until it is induced to dissociate by a nuclear factor in an ATP-dependent manor, thus allowing the guanine nucleotide exchange factor (GEF) RCC1 to mediate exchange of GDP for GTP on Ran (6-7).
- Mattaj, I.W. and Englmeier, L. (1998) Annu Rev Biochem 67, 265-306.
- Kalab, P. et al. (2002) Science 295, 2452-6.
- Becskei, A. and Mattaj, I.W. (2003) Proc Natl Acad Sci USA 100, 1717-22.
- Ribbeck, K. et al. (1998) EMBO J 17, 6587-98.
- Steggerda, S.M. et al. (2000) Mol Biol Cell 11, 703-19.
- Stewart, M. (2000) Cell Struct Funct 25, 217-25.
- Yamada, M. et al. (2004) J Biol Chem 279, 36228-34.
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