Nucleolin (D4C7O) Rabbit mAb #14574
- WB
- IP
- IHC
- IF
- F
- eCLIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
| eCLIP | 1:200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #16864.
Protocol
Specificity / Sensitivity
Nucleolin (D4C7O) Rabbit mAb recognizes endogenous levels of total nucleolin protein. Species reactivity for IHC-P is human only.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly53 of human nucleolin protein.
Background
Nucleolin is a multi-functional protein that is one of the major components of the nucleoli (1). Nucleolin plays an essential role in various steps of ribosome biogenesis including rRNA synthesis, processing of pre-rRNA, pre-ribosomal RNA assembly, and transport of ribosomal proteins out of the nucleus (1-3). While the main function of nucleolin is ribosome biogenesis, it plays an important role in various other nuclear activities. Down regulation of nucleolin leads to increased expression of p53, defects in genome duplication, and a delay at prometaphase during mitosis leading to cell cycle arrest (4-6). In addition, nucleolin has been found in a complex with Rad51 and may participate in DNA repair by homologous recombination (7). Nucleolin binds to the catalytic subunit of the human telomerase reverse transcriptase, hTERT, and is thought to be involved in telomere maintenance (8). Nucleolin also possesses histone chaperone activity and is able to enhance the chromatin remodeling efficiency of SWItch/Sucrose Non Fermentable (SWI/SNF) and ATP-dependent chromatin-assembly factor (ACF), remove histone H2A-H2B dimers from nucleosomes, and facilitate the passage of RNA polymerase through chromatin (9).
- Tajrishi, M.M. et al. (2011) Commun Integr Biol 4, 267-75.
- Ginisty, H. et al. (1999) J Cell Sci 112 ( Pt 6), 761-72.
- Srivastava, M. and Pollard, H.B. (1999) FASEB J 13, 1911-22.
- Takagi, M. et al. (2005) Cell 123, 49-63.
- Ugrinova, I. et al. (2007) BMC Mol Biol 8, 66.
- Ma, N. et al. (2007) J Cell Sci 120, 2091-105.
- De, A. et al. (2006) Biochem Biophys Res Commun 344, 206-13.
- Khurts, S. et al. (2004) J Biol Chem 279, 51508-15.
- Angelov, D. et al. (2006) EMBO J 25, 1669-79.
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