Render Target: STATIC
Render Timestamp: 2024-11-07T09:51:15.582Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-08-01 15:28:09.150
Product last modified at: 2024-05-30T07:06:32.382Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

NUFIP1 Antibody #37783

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 75
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NUFIP1 Antibody recognizes endogenous levels of total NUFIP1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NUFIP1 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    NUFIP1 (nuclear FMRP interacting protein 1) was identified as an RNA-binding protein shuttling between the nucleus and cytoplasm, and it associates with ribosomes in functional synaptoneurosomes (1). Reports have shown that NUFIP1 can function as a receptor for starvation-induced ribophagy to maintain protein homeostasis (2). Ribophagy is a selective form of autophagy targeting ribosomes (reviewed in 3). Lysosomal levels of NUFIP1 were increased upon inhibition of autophagy. Ribophagy was dependent on the ability of ribosome-associated NUFIP1 to interact with LC3B via a conserved LC3-interacting region (LIR) domain and recruit ribosomes to the autophagosome (2). Loss of NUFIP1 or its LC3B binding ability inhibited cell survival following starvation (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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