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OS-9 (D8P4G) Rabbit Monoclonal Antibody #12497

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 83,97
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    OS-9 (D8P4G) Rabbit Monoclonal Antibody recognizes endogenous levels of total OS-9 protein. This antibody reacts with OS-9 isoform 1 (OS-9-1) and OS-9 isoform 2 (OS-9-2).

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Bovine, Horse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human OS-9 protein.

    Background

    Amplified in Osteosarcoma 9 (OS-9) was identified as a gene that is frequently amplified in human sarcomas (1). OS-9 is an N-glycosylated ER resident protein that harbors a mannose-6-phosphate receptor homology (MRH) domain and plays a critical role in mammalian ER quality control, in part, by functioning as a lectin. There are two major isoforms of OS-9 (OS-9-1, OS-9-2) that are generated through alternative splicing of mRNA (2). The expression of both OS-9 isoforms is enhanced upon activation of the IRE1/XBP1 pathway in cells exposed to acute ER stress. It is postulated that OS-9 serves as a retention factor for glycosylated and nonglycosylated, misfolded proteins, preventing their progression through the secretory pathway (3,4). Furthermore, OS-9 promotes the removal of misfolded proteins from the ER lumen and their subsequent proteasome-dependent degradation via its association with the Hrd1-SEL1L ubiquitin ligase complex (5).

    Alternate Names

    Amplified in osteosarcoma 9; endoplasmic reticulum lectin 2; ERLEC2; erlectin 2; OS-9; OS9; OS9 endoplasmic reticulum lectin; osteosarcoma amplified 9, endoplasmic reticulum associated protein; osteosarcoma amplified 9, endoplasmic reticulum lectin; Protein OS-9

    For Research Use Only. Not for Use in Diagnostic Procedures.
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