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60480
p21 Waf1/Cip1 (DCS60) Mouse mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

p21 Waf1/Cip1 (DCS60) Mouse mAb (BSA and Azide Free) #60480

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  1. WB
  2. IHC
Western Blotting Image 1: p21 Waf1/Cip1 (DCS60) Mouse mAb (BSA and Azide Free)
Western blot analysis of extracts from control HeLa cells (lane 1) or p21 Waf1/Cip1 knockout HeLa cells (lane 2) using p21 Waf1/Cip1 (DCS60) Mouse mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The absence of signal in the p21 Waf1/Cip1 knockout HeLa cells confirms the specificity of the antibody for p21 Waf1/Cip1. Data were generated using the standard formulation of this product.
Western Blotting Image 2: p21 Waf1/Cip1 (DCS60) Mouse mAb (BSA and Azide Free)
Western blot analysis of extracts from 293, MCF-7 and COS cells, using p21 Waf1/Cip1 (DCS60) Mouse mAb. Data were generated using the standard formulation of this product.
Western Blotting Image 3: p21 Waf1/Cip1 (DCS60) Mouse mAb (BSA and Azide Free)
Western blot analysis of extracts from 293 and Hela cells, transfected with control (-) or p21 Waf1/Cip1 (+) siRNA. p21 Waf1/Cip1 was detected using p21 Waf1/Cip1 (DCS60) Mouse mAb #2946, p42 MAPK was detected using p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107. The p21 Waf1/Cip1 monoclonal antibody confirms silencing of p21 expression, and the p42 monoclonal antibody is used to control for loading and specificity of p21 Waf1/Cip1 siRNA. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: p21 Waf1/Cip1 (DCS60) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p21 Waf1/Cip1 (DCS60) Mouse mAb. Data were generated using the standard formulation of this product.
To Purchase # 60480
Cat. # Size Qty. Price
60480SF
100 µg
$ 746

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 21
Source/Isotype Mouse IgG2a

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #2946. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #2946.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

p21 Waf1/Cip1 (DCS60) Mouse mAb (BSA and Azide Free) detects endogenous levels of total p21 protein. The antibody does not cross-react with other cdk inhibitors.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human p21 corresponding to the amino-terminal portion of p21.

Background

The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).
  1. Pestell, R.G. et al. (1999) Endocrine Rev. 20, 501-34.
  2. Cheng, J. et al. (1999) EMBO J. 18, 1571-83.
  3. Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-9.
  4. Wang, Y. and Prives, C. (1995) Nature 376, 88-91.
  5. Sheaff, R.J. et al. (2000) Cell 5, 403-10.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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